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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M49 Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP466603FNH1
CSB-EP466603FNH1
CSB-BP466603FNH1
CSB-MP466603FNH1
CSB-EP466603FNH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Peptidase T (pepT) CSB-YP466604FNH
CSB-EP466604FNH
CSB-BP466604FNH
CSB-MP466604FNH
CSB-EP466604FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UPF0342 protein Spy49_0636 (Spy49_0636) CSB-YP466605FNH
CSB-EP466605FNH
CSB-BP466605FNH
CSB-MP466605FNH
CSB-EP466605FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ribosome maturation factor RimM (rimM) CSB-YP466606FNH
CSB-EP466606FNH
CSB-BP466606FNH
CSB-MP466606FNH
CSB-EP466606FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Phosphopentomutase (deoB) CSB-YP466607FNH
CSB-EP466607FNH
CSB-BP466607FNH
CSB-MP466607FNH
CSB-EP466607FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UPF0176 protein Spy49_0726 (Spy49_0726) CSB-YP466608FNH
CSB-EP466608FNH
CSB-BP466608FNH
CSB-MP466608FNH
CSB-EP466608FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L10 (rplJ) CSB-YP466609FNH
CSB-EP466609FNH
CSB-BP466609FNH
CSB-MP466609FNH
CSB-EP466609FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UPF0758 protein Spy49_0870 (Spy49_0870) CSB-YP466610FNH
CSB-EP466610FNH
CSB-BP466610FNH
CSB-MP466610FNH
CSB-EP466610FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UvrABC system protein B (uvrB), partial CSB-YP466611FNH
CSB-EP466611FNH
CSB-BP466611FNH
CSB-MP466611FNH
CSB-EP466611FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 S-adenosylmethionine synthase (metK) CSB-YP466612FNH
CSB-EP466612FNH
CSB-BP466612FNH
CSB-MP466612FNH
CSB-EP466612FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP466613FNH
CSB-EP466613FNH
CSB-BP466613FNH
CSB-MP466613FNH
CSB-EP466613FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Arginine deiminase (arcA) CSB-YP466614FNH
CSB-EP466614FNH
CSB-BP466614FNH
CSB-MP466614FNH
CSB-EP466614FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 S-ribosylhomocysteine lyase (luxS) CSB-YP466615FNH
CSB-EP466615FNH
CSB-BP466615FNH
CSB-MP466615FNH
CSB-EP466615FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP466616FNH
CSB-EP466616FNH
CSB-BP466616FNH
CSB-MP466616FNH
CSB-EP466616FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP466617KBH
CSB-EP466617KBH
CSB-BP466617KBH
CSB-MP466617KBH
CSB-EP466617KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein TusC (tusC) CSB-YP466618KBH
CSB-EP466618KBH
CSB-BP466618KBH
CSB-MP466618KBH
CSB-EP466618KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L4 (rplD) CSB-YP466619KBH
CSB-EP466619KBH
CSB-BP466619KBH
CSB-MP466619KBH
CSB-EP466619KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L18 (rplR) CSB-YP466620KBH
CSB-EP466620KBH
CSB-BP466620KBH
CSB-MP466620KBH
CSB-EP466620KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 3-dehydroquinate dehydratase (aroQ) CSB-YP466621KBH
CSB-EP466621KBH
CSB-BP466621KBH
CSB-MP466621KBH
CSB-EP466621KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP466622KBH
CSB-EP466622KBH
CSB-BP466622KBH
CSB-MP466622KBH
CSB-EP466622KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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