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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aliivibrio salmonicida 30S ribosomal protein S9 (rpsI) CSB-YP467003AZM
CSB-EP467003AZM
CSB-BP467003AZM
CSB-MP467003AZM
CSB-EP467003AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP467004AZM
CSB-EP467004AZM
CSB-BP467004AZM
CSB-MP467004AZM
CSB-EP467004AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP467005AZM
CSB-EP467005AZM
CSB-BP467005AZM
CSB-MP467005AZM
CSB-EP467005AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Shikimate kinase (aroK) CSB-YP467006AZM
CSB-EP467006AZM
CSB-BP467006AZM
CSB-MP467006AZM
CSB-EP467006AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP467007AZM
CSB-EP467007AZM
CSB-BP467007AZM
CSB-MP467007AZM
CSB-EP467007AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 30S ribosomal protein S18 (rpsR) CSB-YP467008AZM
CSB-EP467008AZM
CSB-BP467008AZM
CSB-MP467008AZM
CSB-EP467008AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida DNA mismatch repair protein mutL (mutL), partial CSB-YP467009AZM
CSB-EP467009AZM
CSB-BP467009AZM
CSB-MP467009AZM
CSB-EP467009AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Aspartate carbamoyltransferase (pyrB) CSB-YP467010AZM
CSB-EP467010AZM
CSB-BP467010AZM
CSB-MP467010AZM
CSB-EP467010AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0301 protein VSAL_I0547 (VSAL_I0547) CSB-YP467011AZM
CSB-EP467011AZM
CSB-BP467011AZM
CSB-MP467011AZM
CSB-EP467011AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida RNA pyrophosphohydrolase (rppH) CSB-YP467012AZM
CSB-EP467012AZM
CSB-BP467012AZM
CSB-MP467012AZM
CSB-EP467012AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L20 (rplT) CSB-YP467013AZM
CSB-EP467013AZM
CSB-BP467013AZM
CSB-MP467013AZM
CSB-EP467013AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribosomal protein L11 methyltransferase (prmA) CSB-YP467014AZM
CSB-EP467014AZM
CSB-BP467014AZM
CSB-MP467014AZM
CSB-EP467014AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP467015AZM
CSB-EP467015AZM
CSB-BP467015AZM
CSB-MP467015AZM
CSB-EP467015AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Peptide chain release factor 3 (prfC), partial CSB-YP467016AZM
CSB-EP467016AZM
CSB-BP467016AZM
CSB-MP467016AZM
CSB-EP467016AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Carbon storage regulator homolog (csrA) CSB-YP467017AZM
CSB-EP467017AZM
CSB-BP467017AZM
CSB-MP467017AZM
CSB-EP467017AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L7/L12 (rplL) CSB-YP467018AZM
CSB-EP467018AZM
CSB-BP467018AZM
CSB-MP467018AZM
CSB-EP467018AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP467019AZM
CSB-EP467019AZM
CSB-BP467019AZM
CSB-MP467019AZM
CSB-EP467019AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Membrane protein insertase YidC (yidC), partial CSB-YP467020AZM1
CSB-EP467020AZM1
CSB-BP467020AZM1
CSB-MP467020AZM1
CSB-EP467020AZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP467021AZM
CSB-EP467021AZM
CSB-BP467021AZM
CSB-MP467021AZM
CSB-EP467021AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0758 protein VSAL_I0192 (VSAL_I0192) CSB-YP467022AZM
CSB-EP467022AZM
CSB-BP467022AZM
CSB-MP467022AZM
CSB-EP467022AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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