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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O157:H7 Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP466803EOE
CSB-EP466803EOE
CSB-BP466803EOE
CSB-MP466803EOE
CSB-EP466803EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Small heat shock protein ibpB (ibpB) CSB-YP466804EOE
CSB-EP466804EOE
CSB-BP466804EOE
CSB-MP466804EOE
CSB-EP466804EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribonuclease P protein component (rnpA) CSB-YP466805EOE
CSB-EP466805EOE
CSB-BP466805EOE
CSB-MP466805EOE
CSB-EP466805EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Bifunctional protein GlmU (glmU) CSB-YP466806EOE
CSB-EP466806EOE
CSB-BP466806EOE
CSB-MP466806EOE
CSB-EP466806EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0745 protein YcgL (ycgL) CSB-YP466807EOE
CSB-EP466807EOE
CSB-BP466807EOE
CSB-MP466807EOE
CSB-EP466807EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP466808EOE
CSB-EP466808EOE
CSB-BP466808EOE
CSB-MP466808EOE
CSB-EP466808EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP466809EOE
CSB-EP466809EOE
CSB-BP466809EOE
CSB-MP466809EOE
CSB-EP466809EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD) CSB-YP466810EOE
CSB-EP466810EOE
CSB-BP466810EOE
CSB-MP466810EOE
CSB-EP466810EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0207 protein yfbR (yfbR) CSB-YP466811EOE
CSB-EP466811EOE
CSB-BP466811EOE
CSB-MP466811EOE
CSB-EP466811EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Penicillin-insensitive murein endopeptidase (mepA) CSB-YP466812EOE
CSB-EP466812EOE
CSB-BP466812EOE
CSB-MP466812EOE
CSB-EP466812EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Aspartate--ammonia ligase (asnA) CSB-YP466813EOE
CSB-EP466813EOE
CSB-BP466813EOE
CSB-MP466813EOE
CSB-EP466813EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 HTH-type transcriptional regulator hdfR (hdfR) CSB-YP466814EOE
CSB-EP466814EOE
CSB-BP466814EOE
CSB-MP466814EOE
CSB-EP466814EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 ATP-dependent RNA helicase rhlB (rhlB) CSB-YP466815EOE
CSB-EP466815EOE
CSB-BP466815EOE
CSB-MP466815EOE
CSB-EP466815EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein CyaY (cyaY) CSB-YP466816EOE
CSB-EP466816EOE
CSB-BP466816EOE
CSB-MP466816EOE
CSB-EP466816EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Chaperone protein DnaJ (dnaJ) CSB-YP466817EOE
CSB-EP466817EOE
CSB-BP466817EOE
CSB-MP466817EOE
CSB-EP466817EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Dihydrodipicolinate reductase (dapB) CSB-YP466818EOE
CSB-EP466818EOE
CSB-BP466818EOE
CSB-MP466818EOE
CSB-EP466818EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 L-carnitine/gamma-butyrobetaine antiporter (caiT), partial CSB-YP466819EOE
CSB-EP466819EOE
CSB-BP466819EOE
CSB-MP466819EOE
CSB-EP466819EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable GTP-binding protein EngB (engB) CSB-YP466820EOE
CSB-EP466820EOE
CSB-BP466820EOE
CSB-MP466820EOE
CSB-EP466820EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 L-rhamnose mutarotase (rhaM) CSB-YP466821EOE
CSB-EP466821EOE
CSB-BP466821EOE
CSB-MP466821EOE
CSB-EP466821EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP466822EOE
CSB-EP466822EOE
CSB-BP466822EOE
CSB-MP466822EOE
CSB-EP466822EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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