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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Protein fixA (fixA) CSB-YP467043ENW
CSB-EP467043ENW
CSB-BP467043ENW
CSB-MP467043ENW
CSB-EP467043ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chaperone protein DnaJ (dnaJ) CSB-YP467044ENW
CSB-EP467044ENW
CSB-BP467044ENW
CSB-MP467044ENW
CSB-EP467044ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP467045ENW
CSB-EP467045ENW
CSB-BP467045ENW
CSB-MP467045ENW
CSB-EP467045ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein MraZ (mraZ) CSB-YP467046ENW
CSB-EP467046ENW
CSB-BP467046ENW
CSB-MP467046ENW
CSB-EP467046ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA gyrase inhibitor YacG (yacG) CSB-YP467047ENW
CSB-EP467047ENW
CSB-BP467047ENW
CSB-MP467047ENW
CSB-EP467047ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP467048ENW
CSB-EP467048ENW
CSB-BP467048ENW
CSB-MP467048ENW
CSB-EP467048ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP467049ENW
CSB-EP467049ENW
CSB-BP467049ENW
CSB-MP467049ENW
CSB-EP467049ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribonuclease HII (rnhB) CSB-YP467050ENW
CSB-EP467050ENW
CSB-BP467050ENW
CSB-MP467050ENW
CSB-EP467050ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Recombination-associated protein rdgC (rdgC) CSB-YP467051ENW
CSB-EP467051ENW
CSB-BP467051ENW
CSB-MP467051ENW
CSB-EP467051ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 1-deoxy-D-xylulose-5-phosphate synthase (dxs) CSB-YP467052ENW
CSB-EP467052ENW
CSB-BP467052ENW
CSB-MP467052ENW
CSB-EP467052ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HMP-PP phosphatase (cof) CSB-YP467053ENW
CSB-EP467053ENW
CSB-BP467053ENW
CSB-MP467053ENW
CSB-EP467053ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase CSB-YP467054ENW
CSB-EP467054ENW
CSB-BP467054ENW
CSB-MP467054ENW
CSB-EP467054ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sigma factor-binding protein crl (crl) CSB-YP467055ENW
CSB-EP467055ENW
CSB-BP467055ENW
CSB-MP467055ENW
CSB-EP467055ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Betaine aldehyde dehydrogenase (betB) CSB-YP467056ENW
CSB-EP467056ENW
CSB-BP467056ENW
CSB-MP467056ENW
CSB-EP467056ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Recombination protein RecR (recR) CSB-YP467057ENW
CSB-EP467057ENW
CSB-BP467057ENW
CSB-MP467057ENW
CSB-EP467057ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Bifunctional protein FolD (folD) CSB-YP467058ENW
CSB-EP467058ENW
CSB-BP467058ENW
CSB-MP467058ENW
CSB-EP467058ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UvrABC system protein C (uvrC) CSB-YP467059ENW
CSB-EP467059ENW
CSB-BP467059ENW
CSB-MP467059ENW
CSB-EP467059ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP467060ENW1
CSB-EP467060ENW1
CSB-BP467060ENW1
CSB-MP467060ENW1
CSB-EP467060ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Leucine--tRNA ligase (leuS), partial CSB-YP467061ENW
CSB-EP467061ENW
CSB-BP467061ENW
CSB-MP467061ENW
CSB-EP467061ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-tagatose-1,6-bisphosphate aldolase subunit kbaZ (kbaZ) CSB-YP467062ENW
CSB-EP467062ENW
CSB-BP467062ENW
CSB-MP467062ENW
CSB-EP467062ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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