Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella newport Tryptophan synthase beta chain (trpB) CSB-YP468043SWS
CSB-EP468043SWS
CSB-BP468043SWS
CSB-MP468043SWS
CSB-EP468043SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP468044SWS1
CSB-EP468044SWS1
CSB-BP468044SWS1
CSB-MP468044SWS1
CSB-EP468044SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Transcriptional regulator NanR (nanR) CSB-YP468045SWS
CSB-EP468045SWS
CSB-BP468045SWS
CSB-MP468045SWS
CSB-EP468045SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Xanthine phosphoribosyltransferase (gpt) CSB-YP468046SWQ
CSB-EP468046SWQ
CSB-BP468046SWQ
CSB-MP468046SWQ
CSB-EP468046SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Aspartate--tRNA ligase (aspS), partial CSB-YP468047SWQ
CSB-EP468047SWQ
CSB-BP468047SWQ
CSB-MP468047SWQ
CSB-EP468047SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Flagellar transcriptional regulator FlhD (flhD) CSB-YP468048SWQ
CSB-EP468048SWQ
CSB-BP468048SWQ
CSB-MP468048SWQ
CSB-EP468048SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Transcriptional repressor NrdR (nrdR) CSB-YP468049SWQ
CSB-EP468049SWQ
CSB-BP468049SWQ
CSB-MP468049SWQ
CSB-EP468049SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Protein mtfA (mtfA) CSB-YP468050SWQ
CSB-EP468050SWQ
CSB-BP468050SWQ
CSB-MP468050SWQ
CSB-EP468050SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP468051SWQ
CSB-EP468051SWQ
CSB-BP468051SWQ
CSB-MP468051SWQ
CSB-EP468051SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Phosphopantetheine adenylyltransferase (coaD) CSB-YP468052SWQ
CSB-EP468052SWQ
CSB-BP468052SWQ
CSB-MP468052SWQ
CSB-EP468052SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg HMP-PP phosphatase (cof) CSB-YP468053SWQ
CSB-EP468053SWQ
CSB-BP468053SWQ
CSB-MP468053SWQ
CSB-EP468053SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP468054SWQ
CSB-EP468054SWQ
CSB-BP468054SWQ
CSB-MP468054SWQ
CSB-EP468054SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Multidrug resistance protein MdtB (mdtB), partial CSB-YP468055SWQ
CSB-EP468055SWQ
CSB-BP468055SWQ
CSB-MP468055SWQ
CSB-EP468055SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Chaperone protein TorD (torD) CSB-YP468056SWQ
CSB-EP468056SWQ
CSB-BP468056SWQ
CSB-MP468056SWQ
CSB-EP468056SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Carboxylate-amine ligase YbdK (ybdK) CSB-YP468057SWQ
CSB-EP468057SWQ
CSB-BP468057SWQ
CSB-MP468057SWQ
CSB-EP468057SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0299 membrane protein yohJ (yohJ), partial CSB-YP468058SWQ1
CSB-EP468058SWQ1
CSB-BP468058SWQ1
CSB-MP468058SWQ1
CSB-EP468058SWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0352 protein yejL (yejL) CSB-YP468059SWQ
CSB-EP468059SWQ
CSB-BP468059SWQ
CSB-MP468059SWQ
CSB-EP468059SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 50S ribosomal protein L34 (rpmH) CSB-YP468060SWQ
CSB-EP468060SWQ
CSB-BP468060SWQ
CSB-MP468060SWQ
CSB-EP468060SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg ATP synthase gamma chain (atpG) CSB-YP468061SWQ
CSB-EP468061SWQ
CSB-BP468061SWQ
CSB-MP468061SWQ
CSB-EP468061SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Aspartate--ammonia ligase (asnA) CSB-YP468062SWQ
CSB-EP468062SWQ
CSB-BP468062SWQ
CSB-MP468062SWQ
CSB-EP468062SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>