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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cyanothece sp. 30S ribosomal protein S19 (rpsS) CSB-YP467843EPW
CSB-EP467843EPW
CSB-BP467843EPW
CSB-MP467843EPW
CSB-EP467843EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP467844EPW
CSB-EP467844EPW
CSB-BP467844EPW
CSB-MP467844EPW
CSB-EP467844EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem II reaction center protein Z (psbZ), partial CSB-YP467845EPW1
CSB-EP467845EPW1
CSB-BP467845EPW1
CSB-MP467845EPW1
CSB-EP467845EPW1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. UPF0133 protein PCC8801_2554 (PCC8801_2554) CSB-YP467846EPW
CSB-EP467846EPW
CSB-BP467846EPW
CSB-MP467846EPW
CSB-EP467846EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP467847EPW
CSB-EP467847EPW
CSB-BP467847EPW
CSB-MP467847EPW
CSB-EP467847EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L35 (rpmI) CSB-YP467848EPW
CSB-EP467848EPW
CSB-BP467848EPW
CSB-MP467848EPW
CSB-EP467848EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP467849EPW
CSB-EP467849EPW
CSB-BP467849EPW
CSB-MP467849EPW
CSB-EP467849EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ribosome maturation factor RimM (rimM) CSB-YP467850EPW
CSB-EP467850EPW
CSB-BP467850EPW
CSB-MP467850EPW
CSB-EP467850EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem II reaction center protein J (psbJ), partial CSB-YP467851EPW
CSB-EP467851EPW
CSB-BP467851EPW
CSB-MP467851EPW
CSB-EP467851EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem II reaction center protein H (psbH), partial CSB-YP467852DZZ1
CSB-EP467852DZZ1
CSB-BP467852DZZ1
CSB-MP467852DZZ1
CSB-EP467852DZZ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ribonuclease Z (rnz) CSB-YP467853DZZ
CSB-EP467853DZZ
CSB-BP467853DZZ
CSB-MP467853DZZ
CSB-EP467853DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S10 (rpsJ) CSB-YP467854DZZ
CSB-EP467854DZZ
CSB-BP467854DZZ
CSB-MP467854DZZ
CSB-EP467854DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP467855DZZ
CSB-EP467855DZZ
CSB-BP467855DZZ
CSB-MP467855DZZ
CSB-EP467855DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Putative membrane protein insertion efficiency factor (PCC7424_2840) CSB-YP467856DZZ
CSB-EP467856DZZ
CSB-BP467856DZZ
CSB-MP467856DZZ
CSB-EP467856DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Urease subunit gamma (ureA) CSB-YP467857DZZ
CSB-EP467857DZZ
CSB-BP467857DZZ
CSB-MP467857DZZ
CSB-EP467857DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP467858DZZ
CSB-EP467858DZZ
CSB-BP467858DZZ
CSB-MP467858DZZ
CSB-EP467858DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP467859DZZ
CSB-EP467859DZZ
CSB-BP467859DZZ
CSB-MP467859DZZ
CSB-EP467859DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Cytochrome c biogenesis protein CcsA (ccsA), partial CSB-YP467860DZZ
CSB-EP467860DZZ
CSB-BP467860DZZ
CSB-MP467860DZZ
CSB-EP467860DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Plastocyanin (petE) CSB-YP467861DZZ
CSB-EP467861DZZ
CSB-BP467861DZZ
CSB-MP467861DZZ
CSB-EP467861DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP467862DZZ
CSB-EP467862DZZ
CSB-BP467862DZZ
CSB-MP467862DZZ
CSB-EP467862DZZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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