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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella heidelberg Dihydroxy-acid dehydratase (ilvD), partial CSB-YP468063SWQ
CSB-EP468063SWQ
CSB-BP468063SWQ
CSB-MP468063SWQ
CSB-EP468063SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg LPS-assembly lipoprotein lptE (lptE) CSB-YP468064SWQ
CSB-EP468064SWQ
CSB-BP468064SWQ
CSB-MP468064SWQ
CSB-EP468064SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Glutamine--tRNA ligase (glnS), partial CSB-YP468065SWQ
CSB-EP468065SWQ
CSB-BP468065SWQ
CSB-MP468065SWQ
CSB-EP468065SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 2-keto-3-deoxy-L-rhamnonate aldolase (rhmA) CSB-YP468066SWQ
CSB-EP468066SWQ
CSB-BP468066SWQ
CSB-MP468066SWQ
CSB-EP468066SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP468067SWQ
CSB-EP468067SWQ
CSB-BP468067SWQ
CSB-MP468067SWQ
CSB-EP468067SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP468068SWQ
CSB-EP468068SWQ
CSB-BP468068SWQ
CSB-MP468068SWQ
CSB-EP468068SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0761 membrane protein yihY (yihY), partial CSB-YP468069SWQ1
CSB-EP468069SWQ1
CSB-BP468069SWQ1
CSB-MP468069SWQ1
CSB-EP468069SWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg HTH-type transcriptional activator rhaR (rhaR) CSB-YP468070SWQ
CSB-EP468070SWQ
CSB-BP468070SWQ
CSB-MP468070SWQ
CSB-EP468070SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP468071SWQ
CSB-EP468071SWQ
CSB-BP468071SWQ
CSB-MP468071SWQ
CSB-EP468071SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Catalase-peroxidase (katG), partial CSB-YP468072SWQ
CSB-EP468072SWQ
CSB-BP468072SWQ
CSB-MP468072SWQ
CSB-EP468072SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Thiamine-phosphate synthase (thiE) CSB-YP468073SWQ
CSB-EP468073SWQ
CSB-BP468073SWQ
CSB-MP468073SWQ
CSB-EP468073SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0145 protein ybjQ (ybjQ) CSB-YP468074SWQ
CSB-EP468074SWQ
CSB-BP468074SWQ
CSB-MP468074SWQ
CSB-EP468074SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Cytidylate kinase (cmk) CSB-YP468075SWQ
CSB-EP468075SWQ
CSB-BP468075SWQ
CSB-MP468075SWQ
CSB-EP468075SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP468076SWQ
CSB-EP468076SWQ
CSB-BP468076SWQ
CSB-MP468076SWQ
CSB-EP468076SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg LexA repressor (lexA) CSB-YP468077SWQ
CSB-EP468077SWQ
CSB-BP468077SWQ
CSB-MP468077SWQ
CSB-EP468077SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0319 protein YccT (yccT) CSB-YP468078SWQ
CSB-EP468078SWQ
CSB-BP468078SWQ
CSB-MP468078SWQ
CSB-EP468078SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Autonomous glycyl radical cofactor (grcA) CSB-YP468079SWQ
CSB-EP468079SWQ
CSB-BP468079SWQ
CSB-MP468079SWQ
CSB-EP468079SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP468080SWQ
CSB-EP468080SWQ
CSB-BP468080SWQ
CSB-MP468080SWQ
CSB-EP468080SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Glyoxylate/hydroxypyruvate reductase A (ghrA) CSB-YP468081SWQ
CSB-EP468081SWQ
CSB-BP468081SWQ
CSB-MP468081SWQ
CSB-EP468081SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Dihydroorotase (pyrC) CSB-YP468082SWQ
CSB-EP468082SWQ
CSB-BP468082SWQ
CSB-MP468082SWQ
CSB-EP468082SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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