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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella newport UPF0756 membrane protein YeaL (yeaL), partial CSB-YP468023SWS1
CSB-EP468023SWS1
CSB-BP468023SWS1
CSB-MP468023SWS1
CSB-EP468023SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP468024SWS
CSB-EP468024SWS
CSB-BP468024SWS
CSB-MP468024SWS
CSB-EP468024SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Phosphoglycerol transferase I (mdoB), partial CSB-YP468025SWS1
CSB-EP468025SWS1
CSB-BP468025SWS1
CSB-MP468025SWS1
CSB-EP468025SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP468026SWS
CSB-EP468026SWS
CSB-BP468026SWS
CSB-MP468026SWS
CSB-EP468026SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport NH (3)-dependent NAD (+) synthetase CSB-YP468027SWS
CSB-EP468027SWS
CSB-BP468027SWS
CSB-MP468027SWS
CSB-EP468027SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP468028SWS
CSB-EP468028SWS
CSB-BP468028SWS
CSB-MP468028SWS
CSB-EP468028SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport L-fucose mutarotase (fucU) CSB-YP468029SWS
CSB-EP468029SWS
CSB-BP468029SWS
CSB-MP468029SWS
CSB-EP468029SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Bifunctional protein aas (aas), partial CSB-YP468030SWS1
CSB-EP468030SWS1
CSB-BP468030SWS1
CSB-MP468030SWS1
CSB-EP468030SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Cell division protein ZapA (zapA) CSB-YP468031SWS
CSB-EP468031SWS
CSB-BP468031SWS
CSB-MP468031SWS
CSB-EP468031SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Electron transport complex protein RnfD (rnfD), partial CSB-YP468032SWS
CSB-EP468032SWS
CSB-BP468032SWS
CSB-MP468032SWS
CSB-EP468032SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport DNA replication terminus site-binding protein (tus) CSB-YP468033SWS
CSB-EP468033SWS
CSB-BP468033SWS
CSB-MP468033SWS
CSB-EP468033SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Probable sugar efflux transporter (sotB), partial CSB-YP468034SWS
CSB-EP468034SWS
CSB-BP468034SWS
CSB-MP468034SWS
CSB-EP468034SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Biosynthetic arginine decarboxylase (speA), partial CSB-YP468035SWS
CSB-EP468035SWS
CSB-BP468035SWS
CSB-MP468035SWS
CSB-EP468035SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Protein sprT (sprT) CSB-YP468036SWS
CSB-EP468036SWS
CSB-BP468036SWS
CSB-MP468036SWS
CSB-EP468036SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0114 protein YqhA (yqhA), partial CSB-YP468037SWS1
CSB-EP468037SWS1
CSB-BP468037SWS1
CSB-MP468037SWS1
CSB-EP468037SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP468038SWS1
CSB-EP468038SWS1
CSB-BP468038SWS1
CSB-MP468038SWS1
CSB-EP468038SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP468039SWS
CSB-EP468039SWS
CSB-BP468039SWS
CSB-MP468039SWS
CSB-EP468039SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) CSB-YP468040SWS
CSB-EP468040SWS
CSB-BP468040SWS
CSB-MP468040SWS
CSB-EP468040SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Carnitine operon protein CaiE (caiE) CSB-YP468041SWS
CSB-EP468041SWS
CSB-BP468041SWS
CSB-MP468041SWS
CSB-EP468041SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0283 membrane protein ycjF (ycjF), partial CSB-YP468042SWS1
CSB-EP468042SWS1
CSB-BP468042SWS1
CSB-MP468042SWS1
CSB-EP468042SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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