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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella schwarzengrund Non-specific ribonucleoside hydrolase rihC (rihC) CSB-YP468183SWV
CSB-EP468183SWV
CSB-BP468183SWV
CSB-MP468183SWV
CSB-EP468183SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund RNA polymerase-associated protein RapA (rapA), partial CSB-YP468184SWV
CSB-EP468184SWV
CSB-BP468184SWV
CSB-MP468184SWV
CSB-EP468184SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP468185SWV1
CSB-EP468185SWV1
CSB-BP468185SWV1
CSB-MP468185SWV1
CSB-EP468185SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Shikimate dehydrogenase (aroE) CSB-YP468186SWV
CSB-EP468186SWV
CSB-BP468186SWV
CSB-MP468186SWV
CSB-EP468186SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP468187SWV
CSB-EP468187SWV
CSB-BP468187SWV
CSB-MP468187SWV
CSB-EP468187SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L30 (rpmD) CSB-YP468188SWV
CSB-EP468188SWV
CSB-BP468188SWV
CSB-MP468188SWV
CSB-EP468188SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L5 (rplE) CSB-YP468189SWV
CSB-EP468189SWV
CSB-BP468189SWV
CSB-MP468189SWV
CSB-EP468189SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L16 (rplP) CSB-YP468190SWV
CSB-EP468190SWV
CSB-BP468190SWV
CSB-MP468190SWV
CSB-EP468190SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0270 protein YheU (yheU) CSB-YP468191SWV
CSB-EP468191SWV
CSB-BP468191SWV
CSB-MP468191SWV
CSB-EP468191SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0289 protein yacF (yacF) CSB-YP468192SWV
CSB-EP468192SWV
CSB-BP468192SWV
CSB-MP468192SWV
CSB-EP468192SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP468193SWV
CSB-EP468193SWV
CSB-BP468193SWV
CSB-MP468193SWV
CSB-EP468193SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund [Protein-PII] uridylyltransferase (glnD), partial CSB-YP468194SWV
CSB-EP468194SWV
CSB-BP468194SWV
CSB-MP468194SWV
CSB-EP468194SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Peptidyl-tRNA hydrolase (pth) CSB-YP468195SWV
CSB-EP468195SWV
CSB-BP468195SWV
CSB-MP468195SWV
CSB-EP468195SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Probable septum site-determining protein MinC (minC) CSB-YP468196SWV
CSB-EP468196SWV
CSB-BP468196SWV
CSB-MP468196SWV
CSB-EP468196SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0059 membrane protein yebN (yebN), partial CSB-YP468197SWV1
CSB-EP468197SWV1
CSB-BP468197SWV1
CSB-MP468197SWV1
CSB-EP468197SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund HTH-type transcriptional regulator malT (malT), partial CSB-YP468198SWV
CSB-EP468198SWV
CSB-BP468198SWV
CSB-MP468198SWV
CSB-EP468198SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP468199SWV
CSB-EP468199SWV
CSB-BP468199SWV
CSB-MP468199SWV
CSB-EP468199SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund tRNA (Ile)-lysidine synthase CSB-YP468200SWV
CSB-EP468200SWV
CSB-BP468200SWV
CSB-MP468200SWV
CSB-EP468200SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP468201SWV
CSB-EP468201SWV
CSB-BP468201SWV
CSB-MP468201SWV
CSB-EP468201SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UvrABC system protein C (uvrC), partial CSB-YP468202SWV
CSB-EP468202SWV
CSB-BP468202SWV
CSB-MP468202SWV
CSB-EP468202SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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