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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella schwarzengrund 5'/3'-nucleotidase SurE (surE) CSB-YP468163SWV
CSB-EP468163SWV
CSB-BP468163SWV
CSB-MP468163SWV
CSB-EP468163SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP468164SWV
CSB-EP468164SWV
CSB-BP468164SWV
CSB-MP468164SWV
CSB-EP468164SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0442 protein yjjB (yjjB), partial CSB-YP468165SWV1
CSB-EP468165SWV1
CSB-BP468165SWV1
CSB-MP468165SWV1
CSB-EP468165SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Lipoate-protein ligase A (lplA) CSB-YP468166SWV
CSB-EP468166SWV
CSB-BP468166SWV
CSB-MP468166SWV
CSB-EP468166SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP468167SWV
CSB-EP468167SWV
CSB-BP468167SWV
CSB-MP468167SWV
CSB-EP468167SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP468168SWV
CSB-EP468168SWV
CSB-BP468168SWV
CSB-MP468168SWV
CSB-EP468168SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Lysophospholipid transporter lplT (lplT), partial CSB-YP468169SWV
CSB-EP468169SWV
CSB-BP468169SWV
CSB-MP468169SWV
CSB-EP468169SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0267 protein yqfB (yqfB) CSB-YP468170SWV
CSB-EP468170SWV
CSB-BP468170SWV
CSB-MP468170SWV
CSB-EP468170SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund HTH-type transcriptional repressor PurR (purR) CSB-YP468171SWV
CSB-EP468171SWV
CSB-BP468171SWV
CSB-MP468171SWV
CSB-EP468171SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Electron transport complex protein RnfB (rnfB) CSB-YP468172SWV
CSB-EP468172SWV
CSB-BP468172SWV
CSB-MP468172SWV
CSB-EP468172SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Phosphoglycerate kinase (pgk) CSB-YP468173SWV
CSB-EP468173SWV
CSB-BP468173SWV
CSB-MP468173SWV
CSB-EP468173SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0301 protein yqgE (yqgE) CSB-YP468174SWV
CSB-EP468174SWV
CSB-BP468174SWV
CSB-MP468174SWV
CSB-EP468174SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0257 lipoprotein ynfC (ynfC) CSB-YP468175SWV
CSB-EP468175SWV
CSB-BP468175SWV
CSB-MP468175SWV
CSB-EP468175SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0114 protein YqhA (yqhA), partial CSB-YP468176SWV1
CSB-EP468176SWV1
CSB-BP468176SWV1
CSB-MP468176SWV1
CSB-EP468176SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP468177SWV1
CSB-EP468177SWV1
CSB-BP468177SWV1
CSB-MP468177SWV1
CSB-EP468177SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 5-keto-4-deoxy-D-glucarate aldolase (garL) CSB-YP468178SWV
CSB-EP468178SWV
CSB-BP468178SWV
CSB-MP468178SWV
CSB-EP468178SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) CSB-YP468179SWV
CSB-EP468179SWV
CSB-BP468179SWV
CSB-MP468179SWV
CSB-EP468179SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ribosome maturation factor RimP (rimP) CSB-YP468180SWV
CSB-EP468180SWV
CSB-BP468180SWV
CSB-MP468180SWV
CSB-EP468180SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund GTPase obg (obg) CSB-YP468181SWV
CSB-EP468181SWV
CSB-BP468181SWV
CSB-MP468181SWV
CSB-EP468181SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L13 (rplM) CSB-YP468182SWV
CSB-EP468182SWV
CSB-BP468182SWV
CSB-MP468182SWV
CSB-EP468182SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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