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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Helicobacter pylori Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP469583HUY
CSB-EP469583HUY
CSB-BP469583HUY
CSB-MP469583HUY
CSB-EP469583HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Deoxycytidine triphosphate deaminase (dcd) CSB-YP469584HUY
CSB-EP469584HUY
CSB-BP469584HUY
CSB-MP469584HUY
CSB-EP469584HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP469585HUY
CSB-EP469585HUY
CSB-BP469585HUY
CSB-MP469585HUY
CSB-EP469585HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S12 (rpsL) CSB-YP469586HUY
CSB-EP469586HUY
CSB-BP469586HUY
CSB-MP469586HUY
CSB-EP469586HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori ATP synthase subunit c (atpE), partial CSB-YP469587HUY1
CSB-EP469587HUY1
CSB-BP469587HUY1
CSB-MP469587HUY1
CSB-EP469587HUY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S6 (rpsF) CSB-YP469588HUY
CSB-EP469588HUY
CSB-BP469588HUY
CSB-MP469588HUY
CSB-EP469588HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP469589HUY
CSB-EP469589HUY
CSB-BP469589HUY
CSB-MP469589HUY
CSB-EP469589HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S13 (rpsM) CSB-YP469590HUY
CSB-EP469590HUY
CSB-BP469590HUY
CSB-MP469590HUY
CSB-EP469590HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L14 (rplN) CSB-YP469591HUY
CSB-EP469591HUY
CSB-BP469591HUY
CSB-MP469591HUY
CSB-EP469591HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L2 (rplB) CSB-YP469592HUY
CSB-EP469592HUY
CSB-BP469592HUY
CSB-MP469592HUY
CSB-EP469592HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Isoleucine--tRNA ligase (ileS), partial CSB-YP469593HUY
CSB-EP469593HUY
CSB-BP469593HUY
CSB-MP469593HUY
CSB-EP469593HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Chromosomal replication initiator protein DnaA (dnaA) CSB-YP469594HUY
CSB-EP469594HUY
CSB-BP469594HUY
CSB-MP469594HUY
CSB-EP469594HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP469595HUY
CSB-EP469595HUY
CSB-BP469595HUY
CSB-MP469595HUY
CSB-EP469595HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 60 kDa chaperonin (groL), partial CSB-YP469596HUY
CSB-EP469596HUY
CSB-BP469596HUY
CSB-MP469596HUY
CSB-EP469596HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Urease accessory protein UreE (ureE) CSB-YP469597HUY
CSB-EP469597HUY
CSB-BP469597HUY
CSB-MP469597HUY
CSB-EP469597HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Threonine--tRNA ligase (thrS), partial CSB-YP469598HUY
CSB-EP469598HUY
CSB-BP469598HUY
CSB-MP469598HUY
CSB-EP469598HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Probable transcriptional regulatory protein HPP12_0160 (HPP12_0160) CSB-YP469599HUY
CSB-EP469599HUY
CSB-BP469599HUY
CSB-MP469599HUY
CSB-EP469599HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori S-adenosylmethionine synthase (metK) CSB-YP469600HUY
CSB-EP469600HUY
CSB-BP469600HUY
CSB-MP469600HUY
CSB-EP469600HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Ubiquinone biosynthesis protein coq4, mitochondrial (coq4), partial CSB-YP469601SXT
CSB-EP469601SXT
CSB-BP469601SXT
CSB-MP469601SXT
CSB-EP469601SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Probable endonuclease lcl3 (lcl3), partial CSB-YP469602SXT1
CSB-EP469602SXT1
CSB-BP469602SXT1
CSB-MP469602SXT1
CSB-EP469602SXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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