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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dictyoglomus thermophilum Deoxycytidine triphosphate deaminase (dcd) CSB-YP469043DKI
CSB-EP469043DKI
CSB-BP469043DKI
CSB-MP469043DKI
CSB-EP469043DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum ATP synthase subunit c (atpE), partial CSB-YP469044DKI1
CSB-EP469044DKI1
CSB-BP469044DKI1
CSB-MP469044DKI1
CSB-EP469044DKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Anthranilate phosphoribosyltransferase (trpD) CSB-YP469045DKI
CSB-EP469045DKI
CSB-BP469045DKI
CSB-MP469045DKI
CSB-EP469045DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP469046DKI
CSB-EP469046DKI
CSB-BP469046DKI
CSB-MP469046DKI
CSB-EP469046DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP469047DKI
CSB-EP469047DKI
CSB-BP469047DKI
CSB-MP469047DKI
CSB-EP469047DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L2 (rplB) CSB-YP469048DKI
CSB-EP469048DKI
CSB-BP469048DKI
CSB-MP469048DKI
CSB-EP469048DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L17 (rplQ) CSB-YP469049DKI
CSB-EP469049DKI
CSB-BP469049DKI
CSB-MP469049DKI
CSB-EP469049DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 3-dehydroquinate synthase (aroB) CSB-YP469050DKI
CSB-EP469050DKI
CSB-BP469050DKI
CSB-MP469050DKI
CSB-EP469050DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Protein MraZ (mraZ) CSB-YP469051DKI
CSB-EP469051DKI
CSB-BP469051DKI
CSB-MP469051DKI
CSB-EP469051DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L27 (rpmA) CSB-YP469052DKI
CSB-EP469052DKI
CSB-BP469052DKI
CSB-MP469052DKI
CSB-EP469052DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP469053DKI
CSB-EP469053DKI
CSB-BP469053DKI
CSB-MP469053DKI
CSB-EP469053DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 30S ribosomal protein S6 (rpsF) CSB-YP469054DKI
CSB-EP469054DKI
CSB-BP469054DKI
CSB-MP469054DKI
CSB-EP469054DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L1 (rplA) CSB-YP469055DKI
CSB-EP469055DKI
CSB-BP469055DKI
CSB-MP469055DKI
CSB-EP469055DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP469056DKI
CSB-EP469056DKI
CSB-BP469056DKI
CSB-MP469056DKI
CSB-EP469056DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Phosphate acyltransferase (plsX) CSB-YP469057DKI
CSB-EP469057DKI
CSB-BP469057DKI
CSB-MP469057DKI
CSB-EP469057DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP469058DKI
CSB-EP469058DKI
CSB-BP469058DKI
CSB-MP469058DKI
CSB-EP469058DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP469059DKI
CSB-EP469059DKI
CSB-BP469059DKI
CSB-MP469059DKI
CSB-EP469059DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP469060TMY
CSB-EP469060TMY
CSB-BP469060TMY
CSB-MP469060TMY
CSB-EP469060TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP469061TMY
CSB-EP469061TMY
CSB-BP469061TMY
CSB-MP469061TMY
CSB-EP469061TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii ATP-dependent protease subunit HslV (hslV) CSB-YP469062TMY
CSB-EP469062TMY
CSB-BP469062TMY
CSB-MP469062TMY
CSB-EP469062TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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