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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Regulator of ribonuclease activity A (rraA) CSB-YP469143EOE
CSB-EP469143EOE
CSB-BP469143EOE
CSB-MP469143EOE
CSB-EP469143EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 L-arabinose isomerase (araA) CSB-YP469144EOE
CSB-EP469144EOE
CSB-BP469144EOE
CSB-MP469144EOE
CSB-EP469144EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein MraZ (mraZ) CSB-YP469145EOE
CSB-EP469145EOE
CSB-BP469145EOE
CSB-MP469145EOE
CSB-EP469145EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein translocase subunit SecA (secA), partial CSB-YP469146EOE
CSB-EP469146EOE
CSB-BP469146EOE
CSB-MP469146EOE
CSB-EP469146EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Aspartate 1-decarboxylase (panD) CSB-YP469147EOE
CSB-EP469147EOE
CSB-BP469147EOE
CSB-MP469147EOE
CSB-EP469147EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 GTP cyclohydrolase-2 (ribA) CSB-YP469148EOE
CSB-EP469148EOE
CSB-BP469148EOE
CSB-MP469148EOE
CSB-EP469148EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glutamate--tRNA ligase (gltX) CSB-YP469149EOE
CSB-EP469149EOE
CSB-BP469149EOE
CSB-MP469149EOE
CSB-EP469149EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0370 protein ypfN (ypfN), partial CSB-YP469150EOE
CSB-EP469150EOE
CSB-BP469150EOE
CSB-MP469150EOE
CSB-EP469150EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Acetylglutamate kinase (argB) CSB-YP469151EOE
CSB-EP469151EOE
CSB-BP469151EOE
CSB-MP469151EOE
CSB-EP469151EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Phosphomethylpyrimidine synthase (thiC) CSB-YP469152EOE
CSB-EP469152EOE
CSB-BP469152EOE
CSB-MP469152EOE
CSB-EP469152EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Isocitrate dehydrogenase kinase/phosphatase (aceK) CSB-YP469153EOE
CSB-EP469153EOE
CSB-BP469153EOE
CSB-MP469153EOE
CSB-EP469153EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 [Protein-PII] uridylyltransferase (glnD), partial CSB-YP469154EOE
CSB-EP469154EOE
CSB-BP469154EOE
CSB-MP469154EOE
CSB-EP469154EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0253 protein yaeP (yaeP) CSB-YP469155EOE
CSB-EP469155EOE
CSB-BP469155EOE
CSB-MP469155EOE
CSB-EP469155EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP469156EOE
CSB-EP469156EOE
CSB-BP469156EOE
CSB-MP469156EOE
CSB-EP469156EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein psiE (psiE), partial CSB-YP469157EOE1
CSB-EP469157EOE1
CSB-BP469157EOE1
CSB-MP469157EOE1
CSB-EP469157EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cytochrome c-552 (nrfA) CSB-YP469158EOE
CSB-EP469158EOE
CSB-BP469158EOE
CSB-MP469158EOE
CSB-EP469158EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Uncharacterized protein matC (matC) CSB-YP469159EOE
CSB-EP469159EOE
CSB-BP469159EOE
CSB-MP469159EOE
CSB-EP469159EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein grpE (grpE) CSB-YP469160EOE
CSB-EP469160EOE
CSB-BP469160EOE
CSB-MP469160EOE
CSB-EP469160EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein RecA (recA) CSB-YP469161EOE
CSB-EP469161EOE
CSB-BP469161EOE
CSB-MP469161EOE
CSB-EP469161EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 60 kDa chaperonin (groL) CSB-YP469162EOE
CSB-EP469162EOE
CSB-BP469162EOE
CSB-MP469162EOE
CSB-EP469162EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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