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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Cysteine--tRNA ligase (cysS) CSB-YP469083EOE
CSB-EP469083EOE
CSB-BP469083EOE
CSB-MP469083EOE
CSB-EP469083EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (citX) CSB-YP469084EOE
CSB-EP469084EOE
CSB-BP469084EOE
CSB-MP469084EOE
CSB-EP469084EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Putative phosphotransferase ydiA (ydiA) CSB-YP469085EOE
CSB-EP469085EOE
CSB-BP469085EOE
CSB-MP469085EOE
CSB-EP469085EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0249 protein ChbG (chbG) CSB-YP469086EOE
CSB-EP469086EOE
CSB-BP469086EOE
CSB-MP469086EOE
CSB-EP469086EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 N-succinylarginine dihydrolase (astB) CSB-YP469087EOE
CSB-EP469087EOE
CSB-BP469087EOE
CSB-MP469087EOE
CSB-EP469087EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0235 protein yggU (yggU) CSB-YP469088EOE
CSB-EP469088EOE
CSB-BP469088EOE
CSB-MP469088EOE
CSB-EP469088EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein mgrB (mgrB), partial CSB-YP469089EOE
CSB-EP469089EOE
CSB-BP469089EOE
CSB-MP469089EOE
CSB-EP469089EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 ProP effector (proQ) CSB-YP469090EOE
CSB-EP469090EOE
CSB-BP469090EOE
CSB-MP469090EOE
CSB-EP469090EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 tRNA (mo5U34)-methyltransferase CSB-YP469091EOE
CSB-EP469091EOE
CSB-BP469091EOE
CSB-MP469091EOE
CSB-EP469091EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Multifunctional CCA protein (cca) CSB-YP469092EOE
CSB-EP469092EOE
CSB-BP469092EOE
CSB-MP469092EOE
CSB-EP469092EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Putrescine aminotransferase (patA) CSB-YP469093EOE
CSB-EP469093EOE
CSB-BP469093EOE
CSB-MP469093EOE
CSB-EP469093EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Biotin synthase (bioB) CSB-YP469094EOE
CSB-EP469094EOE
CSB-BP469094EOE
CSB-MP469094EOE
CSB-EP469094EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UvrABC system protein C (uvrC) CSB-YP469095EOE
CSB-EP469095EOE
CSB-BP469095EOE
CSB-MP469095EOE
CSB-EP469095EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0102 protein yraN (yraN) CSB-YP469096EOE
CSB-EP469096EOE
CSB-BP469096EOE
CSB-MP469096EOE
CSB-EP469096EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosome-binding factor A (rbfA) CSB-YP469097EOE
CSB-EP469097EOE
CSB-BP469097EOE
CSB-MP469097EOE
CSB-EP469097EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP469098EOE
CSB-EP469098EOE
CSB-BP469098EOE
CSB-MP469098EOE
CSB-EP469098EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Molecular chaperone Hsp31 and glyoxalase 3 (hchA) CSB-YP469099EOE
CSB-EP469099EOE
CSB-BP469099EOE
CSB-MP469099EOE
CSB-EP469099EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP469100EOE
CSB-EP469100EOE
CSB-BP469100EOE
CSB-MP469100EOE
CSB-EP469100EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP469101EOE1
CSB-EP469101EOE1
CSB-BP469101EOE1
CSB-MP469101EOE1
CSB-EP469101EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L17 (rplQ) CSB-YP469102EOE
CSB-EP469102EOE
CSB-BP469102EOE
CSB-MP469102EOE
CSB-EP469102EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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