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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella schwarzengrund Phosphonoacetaldehyde hydrolase (phnX) CSB-YP470463SWV
CSB-EP470463SWV
CSB-BP470463SWV
CSB-MP470463SWV
CSB-EP470463SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP470464SWV
CSB-EP470464SWV
CSB-BP470464SWV
CSB-MP470464SWV
CSB-EP470464SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0133 protein ybaB (ybaB) CSB-YP470465SWV
CSB-EP470465SWV
CSB-BP470465SWV
CSB-MP470465SWV
CSB-EP470465SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0265 protein yeeX (yeeX) CSB-YP470466SWV
CSB-EP470466SWV
CSB-BP470466SWV
CSB-MP470466SWV
CSB-EP470466SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund D-serine dehydratase (dsdA) CSB-YP470467SWV
CSB-EP470467SWV
CSB-BP470467SWV
CSB-MP470467SWV
CSB-EP470467SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Membrane protein insertase YidC (yidC) CSB-YP470468SWV
CSB-EP470468SWV
CSB-BP470468SWV
CSB-MP470468SWV
CSB-EP470468SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Aspartate--ammonia ligase (asnA) CSB-YP470469SWV
CSB-EP470469SWV
CSB-BP470469SWV
CSB-MP470469SWV
CSB-EP470469SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Multidrug resistance protein MdtB (mdtB), partial CSB-YP470470SWV
CSB-EP470470SWV
CSB-BP470470SWV
CSB-MP470470SWV
CSB-EP470470SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0299 membrane protein yohJ (yohJ) CSB-YP470471SWV
CSB-EP470471SWV
CSB-BP470471SWV
CSB-MP470471SWV
CSB-EP470471SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund ATP-dependent RNA helicase rhlB (rhlB) CSB-YP470472SWV
CSB-EP470472SWV
CSB-BP470472SWV
CSB-MP470472SWV
CSB-EP470472SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP470473SWV
CSB-EP470473SWV
CSB-BP470473SWV
CSB-MP470473SWV
CSB-EP470473SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0250 protein ybeD (ybeD) CSB-YP470474SWV
CSB-EP470474SWV
CSB-BP470474SWV
CSB-MP470474SWV
CSB-EP470474SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Nucleoside triphosphatase nudI (nudI) CSB-YP470475SWV
CSB-EP470475SWV
CSB-BP470475SWV
CSB-MP470475SWV
CSB-EP470475SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP470476SWV
CSB-EP470476SWV
CSB-BP470476SWV
CSB-MP470476SWV
CSB-EP470476SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund D-tyrosyl-tRNA (Tyr) deacylase CSB-YP470477SWV
CSB-EP470477SWV
CSB-BP470477SWV
CSB-MP470477SWV
CSB-EP470477SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund L-rhamnose isomerase (rhaA) CSB-YP470478SWV
CSB-EP470478SWV
CSB-BP470478SWV
CSB-MP470478SWV
CSB-EP470478SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Cell division protein ZapB (zapB) CSB-YP470479SWV
CSB-EP470479SWV
CSB-BP470479SWV
CSB-MP470479SWV
CSB-EP470479SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Leucine--tRNA ligase (leuS), partial CSB-YP470480SWV
CSB-EP470480SWV
CSB-BP470480SWV
CSB-MP470480SWV
CSB-EP470480SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0271 protein YbgL (ybgL) CSB-YP470481SWV
CSB-EP470481SWV
CSB-BP470481SWV
CSB-MP470481SWV
CSB-EP470481SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP470482SWV
CSB-EP470482SWV
CSB-BP470482SWV
CSB-MP470482SWV
CSB-EP470482SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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