Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Anoxybacillus flavithermus Protein sprT-like (Aflv_0202) CSB-YP469683BZO
CSB-EP469683BZO
CSB-BP469683BZO
CSB-MP469683BZO
CSB-EP469683BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 60 kDa chaperonin (groL), partial CSB-YP469684BZO
CSB-EP469684BZO
CSB-BP469684BZO
CSB-MP469684BZO
CSB-EP469684BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus DNA ligase (ligA), partial CSB-YP469685BZO
CSB-EP469685BZO
CSB-BP469685BZO
CSB-MP469685BZO
CSB-EP469685BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Tyrosine recombinase XerC (xerC) CSB-YP469686BZO
CSB-EP469686BZO
CSB-BP469686BZO
CSB-MP469686BZO
CSB-EP469686BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus UPF0122 protein Aflv_1766 (Aflv_1766) CSB-YP469687BZO
CSB-EP469687BZO
CSB-BP469687BZO
CSB-MP469687BZO
CSB-EP469687BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP469688BZO
CSB-EP469688BZO
CSB-BP469688BZO
CSB-MP469688BZO
CSB-EP469688BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Threonine--tRNA ligase (thrS), partial CSB-YP469689BZO
CSB-EP469689BZO
CSB-BP469689BZO
CSB-MP469689BZO
CSB-EP469689BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP469690BZO
CSB-EP469690BZO
CSB-BP469690BZO
CSB-MP469690BZO
CSB-EP469690BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Transcriptional repressor NrdR (nrdR) CSB-YP469691BZO
CSB-EP469691BZO
CSB-BP469691BZO
CSB-MP469691BZO
CSB-EP469691BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Ribonuclease PH (rph) CSB-YP469692BZO
CSB-EP469692BZO
CSB-BP469692BZO
CSB-MP469692BZO
CSB-EP469692BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP469693BZO
CSB-EP469693BZO
CSB-BP469693BZO
CSB-MP469693BZO
CSB-EP469693BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Probable endonuclease 4 (nfo) CSB-YP469694BZO
CSB-EP469694BZO
CSB-BP469694BZO
CSB-MP469694BZO
CSB-EP469694BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Bifunctional protein FolD (folD) CSB-YP469695BZO
CSB-EP469695BZO
CSB-BP469695BZO
CSB-MP469695BZO
CSB-EP469695BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP469696BZO
CSB-EP469696BZO
CSB-BP469696BZO
CSB-MP469696BZO
CSB-EP469696BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP469697BZO
CSB-EP469697BZO
CSB-BP469697BZO
CSB-MP469697BZO
CSB-EP469697BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus GTPase obg (obg) CSB-YP469698BZO
CSB-EP469698BZO
CSB-BP469698BZO
CSB-MP469698BZO
CSB-EP469698BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Uncharacterized methyltransferase Aflv_0758 (Aflv_0758) CSB-YP469699BZO
CSB-EP469699BZO
CSB-BP469699BZO
CSB-MP469699BZO
CSB-EP469699BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S9 (rpsI) CSB-YP469700BZO
CSB-EP469700BZO
CSB-BP469700BZO
CSB-MP469700BZO
CSB-EP469700BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S7 (rpsG) CSB-YP469701BZO
CSB-EP469701BZO
CSB-BP469701BZO
CSB-MP469701BZO
CSB-EP469701BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 50S ribosomal protein L4 (rplD) CSB-YP469702BZO
CSB-EP469702BZO
CSB-BP469702BZO
CSB-MP469702BZO
CSB-EP469702BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>