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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L20 (rplT) CSB-YP470083AWH
CSB-EP470083AWH
CSB-BP470083AWH
CSB-MP470083AWH
CSB-EP470083AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Deoxycytidine triphosphate deaminase (dcd) CSB-YP470084AWH
CSB-EP470084AWH
CSB-BP470084AWH
CSB-MP470084AWH
CSB-EP470084AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Protein grpE (grpE) CSB-YP470085AWH
CSB-EP470085AWH
CSB-BP470085AWH
CSB-MP470085AWH
CSB-EP470085AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP470086AWH
CSB-EP470086AWH
CSB-BP470086AWH
CSB-MP470086AWH
CSB-EP470086AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S16 (rpsP) CSB-YP470087AWH
CSB-EP470087AWH
CSB-BP470087AWH
CSB-MP470087AWH
CSB-EP470087AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP470088AWH
CSB-EP470088AWH
CSB-BP470088AWH
CSB-MP470088AWH
CSB-EP470088AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP470089AWH
CSB-EP470089AWH
CSB-BP470089AWH
CSB-MP470089AWH
CSB-EP470089AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Nitrogenase iron protein (nifH) CSB-YP470090AWH
CSB-EP470090AWH
CSB-BP470090AWH
CSB-MP470090AWH
CSB-EP470090AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP470091AWH
CSB-EP470091AWH
CSB-BP470091AWH
CSB-MP470091AWH
CSB-EP470091AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Rusticyanin (rus) CSB-YP470092AWH
CSB-EP470092AWH
CSB-BP470092AWH
CSB-MP470092AWH
CSB-EP470092AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans ATP synthase subunit c (atpE), partial CSB-YP470093AWH1
CSB-EP470093AWH1
CSB-BP470093AWH1
CSB-MP470093AWH1
CSB-EP470093AWH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP470094AWH
CSB-EP470094AWH
CSB-BP470094AWH
CSB-MP470094AWH
CSB-EP470094AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP470095AWH
CSB-EP470095AWH
CSB-BP470095AWH
CSB-MP470095AWH
CSB-EP470095AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L32 (rpmF) CSB-YP470096AWH
CSB-EP470096AWH
CSB-BP470096AWH
CSB-MP470096AWH
CSB-EP470096AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Serine-protein kinase rsbW (rsbW) CSB-YP470097BQM
CSB-EP470097BQM
CSB-BP470097BQM
CSB-MP470097BQM
CSB-EP470097BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP470098BQM
CSB-EP470098BQM
CSB-BP470098BQM
CSB-MP470098BQM
CSB-EP470098BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable cytosol aminopeptidase (pepA) CSB-YP470099BQM
CSB-EP470099BQM
CSB-BP470099BQM
CSB-MP470099BQM
CSB-EP470099BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP470100BQM
CSB-EP470100BQM
CSB-BP470100BQM
CSB-MP470100BQM
CSB-EP470100BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0753 protein BCAH820_3189 (BCAH820_3189), partial CSB-YP470101BQM
CSB-EP470101BQM
CSB-BP470101BQM
CSB-MP470101BQM
CSB-EP470101BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP470102BQM
CSB-EP470102BQM
CSB-BP470102BQM
CSB-MP470102BQM
CSB-EP470102BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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