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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces japonicus 40S ribosomal protein S0-A (rps0a) CSB-YP469603SXT
CSB-EP469603SXT
CSB-BP469603SXT
CSB-MP469603SXT
CSB-EP469603SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Cytosolic Fe-S cluster assembly factor NAR1 homolog (SJAG_02497), partial CSB-YP469604SXT
CSB-EP469604SXT
CSB-BP469604SXT
CSB-MP469604SXT
CSB-EP469604SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Restriction of telomere capping protein 5 (rtc5) CSB-YP469605SXT
CSB-EP469605SXT
CSB-BP469605SXT
CSB-MP469605SXT
CSB-EP469605SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Pheromone-processing carboxypeptidase kex1 (kex1), partial CSB-YP469606SXT
CSB-EP469606SXT
CSB-BP469606SXT
CSB-MP469606SXT
CSB-EP469606SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae UPF0631 protein (BRAFLDRAFT_231075) CSB-YP469607BVY
CSB-EP469607BVY
CSB-BP469607BVY
CSB-MP469607BVY
CSB-EP469607BVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Ribosome-releasing factor 2, mitochondrial (mef2), partial CSB-YP469608ETM
CSB-EP469608ETM
CSB-BP469608ETM
CSB-MP469608ETM
CSB-EP469608ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Transcription activator of gluconeogenesis PMAA_028970 (PMAA_028970), partial CSB-YP469609ETM
CSB-EP469609ETM
CSB-BP469609ETM
CSB-MP469609ETM
CSB-EP469609ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei U3 small nucleolar RNA-associated protein 25 (utp25), partial CSB-YP469610ETM
CSB-EP469610ETM
CSB-BP469610ETM
CSB-MP469610ETM
CSB-EP469610ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Probable Xaa-Pro aminopeptidase PMAA_074180 (PMAA_074180) CSB-YP469611ETM
CSB-EP469611ETM
CSB-BP469611ETM
CSB-MP469611ETM
CSB-EP469611ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Altered inheritance of mitochondria protein 24, mitochondrial (aim24) CSB-YP469612ETM
CSB-EP469612ETM
CSB-BP469612ETM
CSB-MP469612ETM
CSB-EP469612ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei 40S ribosomal protein S1 (rps1) CSB-YP469613ETM
CSB-EP469613ETM
CSB-BP469613ETM
CSB-MP469613ETM
CSB-EP469613ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Altered inheritance of mitochondria protein 31, mitochondrial (aim31), partial CSB-YP469614ETM1
CSB-EP469614ETM1
CSB-BP469614ETM1
CSB-MP469614ETM1
CSB-EP469614ETM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Vacuolar protein sorting/targeting protein 10 (vps10), partial CSB-YP469615ETM
CSB-EP469615ETM
CSB-BP469615ETM
CSB-MP469615ETM
CSB-EP469615ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Amino-acid acetyltransferase, mitochondrial (arg2), partial CSB-YP469616ETM
CSB-EP469616ETM
CSB-BP469616ETM
CSB-MP469616ETM
CSB-EP469616ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Probable dipeptidyl-aminopeptidase B (dapB), partial CSB-YP469617ETM
CSB-EP469617ETM
CSB-BP469617ETM
CSB-MP469617ETM
CSB-EP469617ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja atra Cathelicidin-NA antimicrobial peptide CSB-YP469618NFG
CSB-EP469618NFG
CSB-BP469618NFG
CSB-MP469618NFG
CSB-EP469618NFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable helicase MAGATAMA 3 (MAA3), partial CSB-YP469619DOA
CSB-EP469619DOA
CSB-BP469619DOA
CSB-MP469619DOA
CSB-EP469619DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Ninja-family protein 5 CSB-YP469620ZAX
CSB-EP469620ZAX
CSB-BP469620ZAX
CSB-MP469620ZAX
CSB-EP469620ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays CASP-like protein 10, partial CSB-YP469621ZAX1
CSB-EP469621ZAX1
CSB-BP469621ZAX1
CSB-MP469621ZAX1
CSB-EP469621ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays CASP-like protein 8, partial CSB-YP469622ZAX1
CSB-EP469622ZAX1
CSB-BP469622ZAX1
CSB-MP469622ZAX1
CSB-EP469622ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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