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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter baumannii DNA replication and repair protein recF (recF) CSB-YP469943AWM
CSB-EP469943AWM
CSB-BP469943AWM
CSB-MP469943AWM
CSB-EP469943AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Protein grpE (grpE) CSB-YP469944AWM
CSB-EP469944AWM
CSB-BP469944AWM
CSB-MP469944AWM
CSB-EP469944AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP469945AWM
CSB-EP469945AWM
CSB-BP469945AWM
CSB-MP469945AWM
CSB-EP469945AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Orotate phosphoribosyltransferase (pyrE) CSB-YP469946AWM
CSB-EP469946AWM
CSB-BP469946AWM
CSB-MP469946AWM
CSB-EP469946AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L28 (rpmB) CSB-YP469947TNG
CSB-EP469947TNG
CSB-BP469947TNG
CSB-MP469947TNG
CSB-EP469947TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP469948TNG
CSB-EP469948TNG
CSB-BP469948TNG
CSB-MP469948TNG
CSB-EP469948TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Probable rRNA maturation factor (THA_1410) CSB-YP469949TNG
CSB-EP469949TNG
CSB-BP469949TNG
CSB-MP469949TNG
CSB-EP469949TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Pseudouridine-5'-phosphate glycosidase (psuG) CSB-YP469950TNG
CSB-EP469950TNG
CSB-BP469950TNG
CSB-MP469950TNG
CSB-EP469950TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Transcription elongation factor GreA (greA) CSB-YP469951TNG
CSB-EP469951TNG
CSB-BP469951TNG
CSB-MP469951TNG
CSB-EP469951TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Phosphoglycerate kinase (pgk) CSB-YP469952TNG
CSB-EP469952TNG
CSB-BP469952TNG
CSB-MP469952TNG
CSB-EP469952TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP469953TNG
CSB-EP469953TNG
CSB-BP469953TNG
CSB-MP469953TNG
CSB-EP469953TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Phosphoglucosamine mutase (glmM) CSB-YP469954TNG
CSB-EP469954TNG
CSB-BP469954TNG
CSB-MP469954TNG
CSB-EP469954TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Probable GTP-binding protein EngB (engB) CSB-YP469955TNG
CSB-EP469955TNG
CSB-BP469955TNG
CSB-MP469955TNG
CSB-EP469955TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Cytidylate kinase (cmk) CSB-YP469956TNG
CSB-EP469956TNG
CSB-BP469956TNG
CSB-MP469956TNG
CSB-EP469956TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Pyrrolidone-carboxylate peptidase (pcp) CSB-YP469957TNG
CSB-EP469957TNG
CSB-BP469957TNG
CSB-MP469957TNG
CSB-EP469957TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus tRNA (guanine-N (1)-)-methyltransferase CSB-YP469958TNG
CSB-EP469958TNG
CSB-BP469958TNG
CSB-MP469958TNG
CSB-EP469958TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus UPF0348 protein THA_149 (THA_149) CSB-YP469959TNG
CSB-EP469959TNG
CSB-BP469959TNG
CSB-MP469959TNG
CSB-EP469959TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Dihydrodipicolinate synthase (dapA) CSB-YP469960TNG
CSB-EP469960TNG
CSB-BP469960TNG
CSB-MP469960TNG
CSB-EP469960TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Proline--tRNA ligase (proS), partial CSB-YP469961TNG
CSB-EP469961TNG
CSB-BP469961TNG
CSB-MP469961TNG
CSB-EP469961TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Tyrosine recombinase XerC (xerC) CSB-YP469962TNG
CSB-EP469962TNG
CSB-BP469962TNG
CSB-MP469962TNG
CSB-EP469962TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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