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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP470003BQP
CSB-EP470003BQP
CSB-BP470003BQP
CSB-MP470003BQP
CSB-EP470003BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uracil phosphoribosyltransferase (upp) CSB-YP470004BQP
CSB-EP470004BQP
CSB-BP470004BQP
CSB-MP470004BQP
CSB-EP470004BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein N (sspN) CSB-YP470005BQP
CSB-EP470005BQP
CSB-BP470005BQP
CSB-MP470005BQP
CSB-EP470005BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP470006BQP
CSB-EP470006BQP
CSB-BP470006BQP
CSB-MP470006BQP
CSB-EP470006BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP470007BQP
CSB-EP470007BQP
CSB-BP470007BQP
CSB-MP470007BQP
CSB-EP470007BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP470008BQP
CSB-EP470008BQP
CSB-BP470008BQP
CSB-MP470008BQP
CSB-EP470008BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Initiation-control protein yabA (BCG9842_B5278) CSB-YP470009BQP
CSB-EP470009BQP
CSB-BP470009BQP
CSB-MP470009BQP
CSB-EP470009BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP470010BQP
CSB-EP470010BQP
CSB-BP470010BQP
CSB-MP470010BQP
CSB-EP470010BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L10 (rplJ) CSB-YP470011BQP
CSB-EP470011BQP
CSB-BP470011BQP
CSB-MP470011BQP
CSB-EP470011BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L4 (rplD) CSB-YP470012BQP
CSB-EP470012BQP
CSB-BP470012BQP
CSB-MP470012BQP
CSB-EP470012BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S17 (rpsQ) CSB-YP470013BQP
CSB-EP470013BQP
CSB-BP470013BQP
CSB-MP470013BQP
CSB-EP470013BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S8 (rpsH) CSB-YP470014BQP
CSB-EP470014BQP
CSB-BP470014BQP
CSB-MP470014BQP
CSB-EP470014BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S11 (rpsK) CSB-YP470015BQP
CSB-EP470015BQP
CSB-BP470015BQP
CSB-MP470015BQP
CSB-EP470015BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NH (3)-dependent NAD (+) synthetase CSB-YP470016BQP
CSB-EP470016BQP
CSB-BP470016BQP
CSB-MP470016BQP
CSB-EP470016BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP470017BQP
CSB-EP470017BQP
CSB-BP470017BQP
CSB-MP470017BQP
CSB-EP470017BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein sprT-like (BCG9842_B5054) CSB-YP470018BQP
CSB-EP470018BQP
CSB-BP470018BQP
CSB-MP470018BQP
CSB-EP470018BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosome maturation factor RimP (rimP) CSB-YP470019BQP
CSB-EP470019BQP
CSB-BP470019BQP
CSB-MP470019BQP
CSB-EP470019BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP470020BQP
CSB-EP470020BQP
CSB-BP470020BQP
CSB-MP470020BQP
CSB-EP470020BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L19 (rplS) CSB-YP470021BQP
CSB-EP470021BQP
CSB-BP470021BQP
CSB-MP470021BQP
CSB-EP470021BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0296 protein BCG9842_B1272 (BCG9842_B1272) CSB-YP470022BQP
CSB-EP470022BQP
CSB-BP470022BQP
CSB-MP470022BQP
CSB-EP470022BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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