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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Ribonuclease Z (rnz) CSB-YP470143BQM
CSB-EP470143BQM
CSB-BP470143BQM
CSB-MP470143BQM
CSB-EP470143BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 10 kDa chaperonin (groS) CSB-YP470144BQM
CSB-EP470144BQM
CSB-BP470144BQM
CSB-MP470144BQM
CSB-EP470144BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP470145BQM
CSB-EP470145BQM
CSB-BP470145BQM
CSB-MP470145BQM
CSB-EP470145BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP470146BQM
CSB-EP470146BQM
CSB-BP470146BQM
CSB-MP470146BQM
CSB-EP470146BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP470147BQM
CSB-EP470147BQM
CSB-BP470147BQM
CSB-MP470147BQM
CSB-EP470147BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chaperone protein DnaJ (dnaJ) CSB-YP470148BQM
CSB-EP470148BQM
CSB-BP470148BQM
CSB-MP470148BQM
CSB-EP470148BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Arginine deiminase (arcA) CSB-YP470149BQM
CSB-EP470149BQM
CSB-BP470149BQM
CSB-MP470149BQM
CSB-EP470149BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP470150BQM
CSB-EP470150BQM
CSB-BP470150BQM
CSB-MP470150BQM
CSB-EP470150BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Shikimate dehydrogenase (aroE) CSB-YP470151BQM
CSB-EP470151BQM
CSB-BP470151BQM
CSB-MP470151BQM
CSB-EP470151BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Inosose dehydratase (iolE) CSB-YP470152BQM
CSB-EP470152BQM
CSB-BP470152BQM
CSB-MP470152BQM
CSB-EP470152BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L21 (rplU) CSB-YP470153BQM
CSB-EP470153BQM
CSB-BP470153BQM
CSB-MP470153BQM
CSB-EP470153BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP470154BQM
CSB-EP470154BQM
CSB-BP470154BQM
CSB-MP470154BQM
CSB-EP470154BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative metal-dependent hydrolase BCAH820_2714 (BCAH820_2714) CSB-YP470155BQM
CSB-EP470155BQM
CSB-BP470155BQM
CSB-MP470155BQM
CSB-EP470155BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase A chain (kdpA), partial CSB-YP470156BQM
CSB-EP470156BQM
CSB-BP470156BQM
CSB-MP470156BQM
CSB-EP470156BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0756 membrane protein BCAH820_4710 (BCAH820_4710), partial CSB-YP470157BQM1
CSB-EP470157BQM1
CSB-BP470157BQM1
CSB-MP470157BQM1
CSB-EP470157BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0173 metal-dependent hydrolase BCAH820_4729 (BCAH820_4729) CSB-YP470158BQM
CSB-EP470158BQM
CSB-BP470158BQM
CSB-MP470158BQM
CSB-EP470158BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Large-conductance mechanosensitive channel (mscL), partial CSB-YP470159BQM1
CSB-EP470159BQM1
CSB-BP470159BQM1
CSB-MP470159BQM1
CSB-EP470159BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCAH820_0960) CSB-YP470160BQM
CSB-EP470160BQM
CSB-BP470160BQM
CSB-MP470160BQM
CSB-EP470160BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Leucine--tRNA ligase (leuS), partial CSB-YP470161BQM
CSB-EP470161BQM
CSB-BP470161BQM
CSB-MP470161BQM
CSB-EP470161BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative membrane protein insertion efficiency factor (BCAH820_4913) CSB-YP470162BQM
CSB-EP470162BQM
CSB-BP470162BQM
CSB-MP470162BQM
CSB-EP470162BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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