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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermosipho africanus Ribosomal protein L11 methyltransferase (prmA) CSB-YP469963TNG
CSB-EP469963TNG
CSB-BP469963TNG
CSB-MP469963TNG
CSB-EP469963TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP469964TNG
CSB-EP469964TNG
CSB-BP469964TNG
CSB-MP469964TNG
CSB-EP469964TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP469965TNG
CSB-EP469965TNG
CSB-BP469965TNG
CSB-MP469965TNG
CSB-EP469965TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Flagellar assembly factor FliW (fliW) CSB-YP469966TNG
CSB-EP469966TNG
CSB-BP469966TNG
CSB-MP469966TNG
CSB-EP469966TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus D-ribose pyranase (rbsD) CSB-YP469967TNG
CSB-EP469967TNG
CSB-BP469967TNG
CSB-MP469967TNG
CSB-EP469967TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Phosphate acyltransferase (plsX) CSB-YP469968TNG
CSB-EP469968TNG
CSB-BP469968TNG
CSB-MP469968TNG
CSB-EP469968TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus DNA mismatch repair protein mutL (mutL), partial CSB-YP469969TNG
CSB-EP469969TNG
CSB-BP469969TNG
CSB-MP469969TNG
CSB-EP469969TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP469970TNG
CSB-EP469970TNG
CSB-BP469970TNG
CSB-MP469970TNG
CSB-EP469970TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP469971TNG
CSB-EP469971TNG
CSB-BP469971TNG
CSB-MP469971TNG
CSB-EP469971TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L22 (rplV) CSB-YP469972TNG
CSB-EP469972TNG
CSB-BP469972TNG
CSB-MP469972TNG
CSB-EP469972TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L14 (rplN) CSB-YP469973TNG
CSB-EP469973TNG
CSB-BP469973TNG
CSB-MP469973TNG
CSB-EP469973TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 30S ribosomal protein S5 (rpsE) CSB-YP469974TNG
CSB-EP469974TNG
CSB-BP469974TNG
CSB-MP469974TNG
CSB-EP469974TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 30S ribosomal protein S11 (rpsK) CSB-YP469975TNG
CSB-EP469975TNG
CSB-BP469975TNG
CSB-MP469975TNG
CSB-EP469975TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase B chain (kdpB), partial CSB-YP469976BQP1
CSB-EP469976BQP1
CSB-BP469976BQP1
CSB-MP469976BQP1
CSB-EP469976BQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0342 protein BCG9842_B4422 (BCG9842_B4422) CSB-YP469977BQP
CSB-EP469977BQP
CSB-BP469977BQP
CSB-MP469977BQP
CSB-EP469977BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP469978BQP
CSB-EP469978BQP
CSB-BP469978BQP
CSB-MP469978BQP
CSB-EP469978BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GTPase obg (obg) CSB-YP469979BQP
CSB-EP469979BQP
CSB-BP469979BQP
CSB-MP469979BQP
CSB-EP469979BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Septum formation protein Maf (maf) CSB-YP469980BQP
CSB-EP469980BQP
CSB-BP469980BQP
CSB-MP469980BQP
CSB-EP469980BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamyl-tRNA reductase (hemA) CSB-YP469981BQP
CSB-EP469981BQP
CSB-BP469981BQP
CSB-MP469981BQP
CSB-EP469981BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UvrABC system protein C (uvrC) CSB-YP469982BQP
CSB-EP469982BQP
CSB-BP469982BQP
CSB-MP469982BQP
CSB-EP469982BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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