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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Aspartate carbamoyltransferase (pyrB) CSB-YP470023BQP
CSB-EP470023BQP
CSB-BP470023BQP
CSB-MP470023BQP
CSB-EP470023BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP470024BQP
CSB-EP470024BQP
CSB-BP470024BQP
CSB-MP470024BQP
CSB-EP470024BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP470025BQP
CSB-EP470025BQP
CSB-BP470025BQP
CSB-MP470025BQP
CSB-EP470025BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA ligase (ligA), partial CSB-YP470026BQP
CSB-EP470026BQP
CSB-BP470026BQP
CSB-MP470026BQP
CSB-EP470026BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP470027BQP
CSB-EP470027BQP
CSB-BP470027BQP
CSB-MP470027BQP
CSB-EP470027BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0637 protein BCG9842_B1177 (BCG9842_B1177) CSB-YP470028BQP
CSB-EP470028BQP
CSB-BP470028BQP
CSB-MP470028BQP
CSB-EP470028BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N-acetyldiaminopimelate deacetylase (BCG9842_B1157) CSB-YP470029BQP
CSB-EP470029BQP
CSB-BP470029BQP
CSB-MP470029BQP
CSB-EP470029BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0374 protein BCG9842_B4794 (BCG9842_B4794) CSB-YP470030BQP
CSB-EP470030BQP
CSB-BP470030BQP
CSB-MP470030BQP
CSB-EP470030BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP470031BQP
CSB-EP470031BQP
CSB-BP470031BQP
CSB-MP470031BQP
CSB-EP470031BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphopentomutase (deoB) CSB-YP470032BQP
CSB-EP470032BQP
CSB-BP470032BQP
CSB-MP470032BQP
CSB-EP470032BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP470033BQP
CSB-EP470033BQP
CSB-BP470033BQP
CSB-MP470033BQP
CSB-EP470033BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0229 protein BCG9842_B4751 (BCG9842_B4751) CSB-YP470034BQP
CSB-EP470034BQP
CSB-BP470034BQP
CSB-MP470034BQP
CSB-EP470034BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable butyrate kinase (buk) CSB-YP470035BQP
CSB-EP470035BQP
CSB-BP470035BQP
CSB-MP470035BQP
CSB-EP470035BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thiazole synthase (thiG) CSB-YP470036BQP
CSB-EP470036BQP
CSB-BP470036BQP
CSB-MP470036BQP
CSB-EP470036BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chaperone protein DnaJ (dnaJ) CSB-YP470037BQP
CSB-EP470037BQP
CSB-BP470037BQP
CSB-MP470037BQP
CSB-EP470037BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP470038BQP
CSB-EP470038BQP
CSB-BP470038BQP
CSB-MP470038BQP
CSB-EP470038BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative Holliday junction resolvase (BCG9842_B0730) CSB-YP470039BQP
CSB-EP470039BQP
CSB-BP470039BQP
CSB-MP470039BQP
CSB-EP470039BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP470040BUG
CSB-EP470040BUG
CSB-BP470040BUG
CSB-MP470040BUG
CSB-EP470040BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi GTPase obg (obg) CSB-YP470041BUG
CSB-EP470041BUG
CSB-BP470041BUG
CSB-MP470041BUG
CSB-EP470041BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi DNA mismatch repair protein MutS (mutS), partial CSB-YP470042BUG
CSB-EP470042BUG
CSB-BP470042BUG
CSB-MP470042BUG
CSB-EP470042BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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