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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cyanothece sp. Adenylyl-sulfate kinase (cysC) CSB-YP470203DZZ
CSB-EP470203DZZ
CSB-BP470203DZZ
CSB-MP470203DZZ
CSB-EP470203DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. DNA ligase (ligA), partial CSB-YP470204DZZ
CSB-EP470204DZZ
CSB-BP470204DZZ
CSB-MP470204DZZ
CSB-EP470204DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Urease accessory protein UreF (ureF) CSB-YP470205DZZ
CSB-EP470205DZZ
CSB-BP470205DZZ
CSB-MP470205DZZ
CSB-EP470205DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. D-alanine--D-alanine ligase (ddl) CSB-YP470206DZZ
CSB-EP470206DZZ
CSB-BP470206DZZ
CSB-MP470206DZZ
CSB-EP470206DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem II reaction center Psb28 protein (psb28) CSB-YP470207DZZ
CSB-EP470207DZZ
CSB-BP470207DZZ
CSB-MP470207DZZ
CSB-EP470207DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Cytochrome b6-f complex subunit 7 (petM), partial CSB-YP470208DZZ
CSB-EP470208DZZ
CSB-BP470208DZZ
CSB-MP470208DZZ
CSB-EP470208DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S15 (rpsO) CSB-YP470209DZZ
CSB-EP470209DZZ
CSB-BP470209DZZ
CSB-MP470209DZZ
CSB-EP470209DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Putative Holliday junction resolvase (PCC7424_3098) CSB-YP470210DZZ
CSB-EP470210DZZ
CSB-BP470210DZZ
CSB-MP470210DZZ
CSB-EP470210DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP470211DZZ
CSB-EP470211DZZ
CSB-BP470211DZZ
CSB-MP470211DZZ
CSB-EP470211DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii GTPase obg (obg1) CSB-YP470212AZX
CSB-EP470212AZX
CSB-BP470212AZX
CSB-MP470212AZX
CSB-EP470212AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP470213AZX
CSB-EP470213AZX
CSB-BP470213AZX
CSB-MP470213AZX
CSB-EP470213AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L2 (rplB1) CSB-YP470214AZX
CSB-EP470214AZX
CSB-BP470214AZX
CSB-MP470214AZX
CSB-EP470214AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP470215AZX
CSB-EP470215AZX
CSB-BP470215AZX
CSB-MP470215AZX
CSB-EP470215AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Probable rRNA maturation factor (MADE_1007145) CSB-YP470216AZX
CSB-EP470216AZX
CSB-BP470216AZX
CSB-MP470216AZX
CSB-EP470216AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Adenylosuccinate synthetase (purA) CSB-YP470217AZX
CSB-EP470217AZX
CSB-BP470217AZX
CSB-MP470217AZX
CSB-EP470217AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii LexA repressor (lexA1) CSB-YP470218AZX
CSB-EP470218AZX
CSB-BP470218AZX
CSB-MP470218AZX
CSB-EP470218AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L28 (rpmB) CSB-YP470219AZX
CSB-EP470219AZX
CSB-BP470219AZX
CSB-MP470219AZX
CSB-EP470219AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Probable Fe (2+)-trafficking protein CSB-YP470220AZX
CSB-EP470220AZX
CSB-BP470220AZX
CSB-MP470220AZX
CSB-EP470220AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Catalase-peroxidase (katG), partial CSB-YP470221EYX
CSB-EP470221EYX
CSB-BP470221EYX
CSB-MP470221EYX
CSB-EP470221EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP470222EYX
CSB-EP470222EYX
CSB-BP470222EYX
CSB-MP470222EYX
CSB-EP470222EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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