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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cyanothece sp. N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP470163EPW
CSB-EP470163EPW
CSB-BP470163EPW
CSB-MP470163EPW
CSB-EP470163EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S7 (rpsG) CSB-YP470164EPW
CSB-EP470164EPW
CSB-BP470164EPW
CSB-MP470164EPW
CSB-EP470164EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem II reaction center protein M (psbM), partial CSB-YP470165EPW
CSB-EP470165EPW
CSB-BP470165EPW
CSB-MP470165EPW
CSB-EP470165EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Phosphoglycerate kinase (pgk) CSB-YP470166EPW
CSB-EP470166EPW
CSB-BP470166EPW
CSB-MP470166EPW
CSB-EP470166EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Elongation factor P (efp) CSB-YP470167EPW
CSB-EP470167EPW
CSB-BP470167EPW
CSB-MP470167EPW
CSB-EP470167EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L33 (rpmG) CSB-YP470168EPW
CSB-EP470168EPW
CSB-BP470168EPW
CSB-MP470168EPW
CSB-EP470168EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Trigger factor (tig) CSB-YP470169EPW
CSB-EP470169EPW
CSB-BP470169EPW
CSB-MP470169EPW
CSB-EP470169EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L7/L12 (rplL) CSB-YP470170EPW
CSB-EP470170EPW
CSB-BP470170EPW
CSB-MP470170EPW
CSB-EP470170EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP470171EPW
CSB-EP470171EPW
CSB-BP470171EPW
CSB-MP470171EPW
CSB-EP470171EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem II reaction center protein T (psbT), partial CSB-YP470172EPW
CSB-EP470172EPW
CSB-BP470172EPW
CSB-MP470172EPW
CSB-EP470172EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S6 (rpsF) CSB-YP470173EPW
CSB-EP470173EPW
CSB-BP470173EPW
CSB-MP470173EPW
CSB-EP470173EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP470174EPW
CSB-EP470174EPW
CSB-BP470174EPW
CSB-MP470174EPW
CSB-EP470174EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem II reaction center protein I (psbI), partial CSB-YP470175EPW
CSB-EP470175EPW
CSB-BP470175EPW
CSB-MP470175EPW
CSB-EP470175EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Peptide chain release factor 3 (prfC), partial CSB-YP470176EPW
CSB-EP470176EPW
CSB-BP470176EPW
CSB-MP470176EPW
CSB-EP470176EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP470177EPW
CSB-EP470177EPW
CSB-BP470177EPW
CSB-MP470177EPW
CSB-EP470177EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. D-alanine--D-alanine ligase (ddl) CSB-YP470178EPW
CSB-EP470178EPW
CSB-BP470178EPW
CSB-MP470178EPW
CSB-EP470178EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. PKHD-type hydroxylase PCC8801_2196 (PCC8801_2196) CSB-YP470179EPW
CSB-EP470179EPW
CSB-BP470179EPW
CSB-MP470179EPW
CSB-EP470179EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP470180EPW
CSB-EP470180EPW
CSB-BP470180EPW
CSB-MP470180EPW
CSB-EP470180EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP470181EPW
CSB-EP470181EPW
CSB-BP470181EPW
CSB-MP470181EPW
CSB-EP470181EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. DNA replication and repair protein recF (recF) CSB-YP470182EPW
CSB-EP470182EPW
CSB-BP470182EPW
CSB-MP470182EPW
CSB-EP470182EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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