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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella heidelberg Flavoprotein wrbA (wrbA) CSB-YP470423SWQ
CSB-EP470423SWQ
CSB-BP470423SWQ
CSB-MP470423SWQ
CSB-EP470423SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg N-methyl-L-tryptophan oxidase (solA) CSB-YP470424SWQ
CSB-EP470424SWQ
CSB-BP470424SWQ
CSB-MP470424SWQ
CSB-EP470424SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Glutamate--cysteine ligase (gshA), partial CSB-YP470425SWQ
CSB-EP470425SWQ
CSB-BP470425SWQ
CSB-MP470425SWQ
CSB-EP470425SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Elongation factor P (efp) CSB-YP470426SWQ
CSB-EP470426SWQ
CSB-BP470426SWQ
CSB-MP470426SWQ
CSB-EP470426SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP470427SWQ
CSB-EP470427SWQ
CSB-BP470427SWQ
CSB-MP470427SWQ
CSB-EP470427SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg L-ribulose-5-phosphate 3-epimerase UlaE (ulaE) CSB-YP470428SWQ
CSB-EP470428SWQ
CSB-BP470428SWQ
CSB-MP470428SWQ
CSB-EP470428SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg High frequency lysogenization protein HflD homolog (hflD) CSB-YP470429SWQ
CSB-EP470429SWQ
CSB-BP470429SWQ
CSB-MP470429SWQ
CSB-EP470429SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Cell division protein FtsB (ftsB), partial CSB-YP470430SWQ
CSB-EP470430SWQ
CSB-BP470430SWQ
CSB-MP470430SWQ
CSB-EP470430SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP470431SWQ
CSB-EP470431SWQ
CSB-BP470431SWQ
CSB-MP470431SWQ
CSB-EP470431SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Arginine N-succinyltransferase (astA) CSB-YP470432SWQ
CSB-EP470432SWQ
CSB-BP470432SWQ
CSB-MP470432SWQ
CSB-EP470432SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Putative phosphotransferase ydiA (ydiA) CSB-YP470433SWQ
CSB-EP470433SWQ
CSB-BP470433SWQ
CSB-MP470433SWQ
CSB-EP470433SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP470434SWQ
CSB-EP470434SWQ
CSB-BP470434SWQ
CSB-MP470434SWQ
CSB-EP470434SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Lysine--tRNA ligase (lysS), partial CSB-YP470435SWQ
CSB-EP470435SWQ
CSB-BP470435SWQ
CSB-MP470435SWQ
CSB-EP470435SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Peptide chain release factor 3 (prfC), partial CSB-YP470436SWQ
CSB-EP470436SWQ
CSB-BP470436SWQ
CSB-MP470436SWQ
CSB-EP470436SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Lipoate-protein ligase A (lplA) CSB-YP470437SWQ
CSB-EP470437SWQ
CSB-BP470437SWQ
CSB-MP470437SWQ
CSB-EP470437SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP470438SWQ
CSB-EP470438SWQ
CSB-BP470438SWQ
CSB-MP470438SWQ
CSB-EP470438SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0149 protein ygfB (ygfB) CSB-YP470439SWQ
CSB-EP470439SWQ
CSB-BP470439SWQ
CSB-MP470439SWQ
CSB-EP470439SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg S-adenosylmethionine synthase (metK) CSB-YP470440SWQ
CSB-EP470440SWQ
CSB-BP470440SWQ
CSB-MP470440SWQ
CSB-EP470440SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0257 lipoprotein ynfC (ynfC) CSB-YP470441SWQ
CSB-EP470441SWQ
CSB-BP470441SWQ
CSB-MP470441SWQ
CSB-EP470441SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP470442SWQ
CSB-EP470442SWQ
CSB-BP470442SWQ
CSB-MP470442SWQ
CSB-EP470442SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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