Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermococcus onnurineus Peptidyl-tRNA hydrolase (pth) CSB-YP472025TMR
CSB-EP472025TMR
CSB-BP472025TMR
CSB-MP472025TMR
CSB-EP472025TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus DNA-directed RNA polymerase subunit L (rpoL) CSB-YP472026TMR
CSB-EP472026TMR
CSB-BP472026TMR
CSB-MP472026TMR
CSB-EP472026TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Translation initiation factor 5A (eIF5A) CSB-YP472027TMR
CSB-EP472027TMR
CSB-BP472027TMR
CSB-MP472027TMR
CSB-EP472027TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L37Ae (rpl37Ae) CSB-YP472028TMR
CSB-EP472028TMR
CSB-BP472028TMR
CSB-MP472028TMR
CSB-EP472028TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP472029TMR
CSB-EP472029TMR
CSB-BP472029TMR
CSB-MP472029TMR
CSB-EP472029TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Putative adenylate kinase (TON_0562) CSB-YP472030TMR
CSB-EP472030TMR
CSB-BP472030TMR
CSB-MP472030TMR
CSB-EP472030TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus UPF0235 protein TON_0641 (TON_0641) CSB-YP472031TMR
CSB-EP472031TMR
CSB-BP472031TMR
CSB-MP472031TMR
CSB-EP472031TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus V-type ATP synthase subunit D (atpD) CSB-YP472032TMR
CSB-EP472032TMR
CSB-BP472032TMR
CSB-MP472032TMR
CSB-EP472032TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable deoxyhypusine synthase (dys) CSB-YP472033TMR
CSB-EP472033TMR
CSB-BP472033TMR
CSB-MP472033TMR
CSB-EP472033TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Ribonuclease HII (rnhB) CSB-YP472034TMR
CSB-EP472034TMR
CSB-BP472034TMR
CSB-MP472034TMR
CSB-EP472034TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus DNA polymerase sliding clamp (pcn) CSB-YP472035TMR
CSB-EP472035TMR
CSB-BP472035TMR
CSB-MP472035TMR
CSB-EP472035TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Preprotein translocase subunit secG (secG), partial CSB-YP472036TMR
CSB-EP472036TMR
CSB-BP472036TMR
CSB-MP472036TMR
CSB-EP472036TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Adenylosuccinate synthetase (purA) CSB-YP472037TMR
CSB-EP472037TMR
CSB-BP472037TMR
CSB-MP472037TMR
CSB-EP472037TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L24e (rpl24e) CSB-YP472038TMR
CSB-EP472038TMR
CSB-BP472038TMR
CSB-MP472038TMR
CSB-EP472038TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus UPF0271 protein TON_1051 (TON_1051) CSB-YP472039TMR
CSB-EP472039TMR
CSB-BP472039TMR
CSB-MP472039TMR
CSB-EP472039TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein LX (rplX) CSB-YP472040TMR
CSB-EP472040TMR
CSB-BP472040TMR
CSB-MP472040TMR
CSB-EP472040TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP472041TMR
CSB-EP472041TMR
CSB-BP472041TMR
CSB-MP472041TMR
CSB-EP472041TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP472042TMR
CSB-EP472042TMR
CSB-BP472042TMR
CSB-MP472042TMR
CSB-EP472042TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Double-stranded RNA-binding protein 5 (DRB5) CSB-YP472043OFG
CSB-EP472043OFG
CSB-BP472043OFG
CSB-MP472043OFG
CSB-EP472043OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica B3 domain-containing protein Os03g0164300 (Os03g0164300, LOC_Os03g06850) CSB-YP472044OFG
CSB-EP472044OFG
CSB-BP472044OFG
CSB-MP472044OFG
CSB-EP472044OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>