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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP472225BQL
CSB-EP472225BQL
CSB-BP472225BQL
CSB-MP472225BQL
CSB-EP472225BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphopentomutase (deoB) CSB-YP472226BQL
CSB-EP472226BQL
CSB-BP472226BQL
CSB-MP472226BQL
CSB-EP472226BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP472227BQL
CSB-EP472227BQL
CSB-BP472227BQL
CSB-MP472227BQL
CSB-EP472227BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP472228BQL
CSB-EP472228BQL
CSB-BP472228BQL
CSB-MP472228BQL
CSB-EP472228BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Arginine repressor (argR) CSB-YP472229BQL
CSB-EP472229BQL
CSB-BP472229BQL
CSB-MP472229BQL
CSB-EP472229BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N utilization substance protein B homolog (nusB) CSB-YP472230BQL
CSB-EP472230BQL
CSB-BP472230BQL
CSB-MP472230BQL
CSB-EP472230BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Shikimate kinase (aroK) CSB-YP472231BQL
CSB-EP472231BQL
CSB-BP472231BQL
CSB-MP472231BQL
CSB-EP472231BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP472232BQL
CSB-EP472232BQL
CSB-BP472232BQL
CSB-MP472232BQL
CSB-EP472232BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S20 (rpsT) CSB-YP472233BQL
CSB-EP472233BQL
CSB-BP472233BQL
CSB-MP472233BQL
CSB-EP472233BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP472234BQL
CSB-EP472234BQL
CSB-BP472234BQL
CSB-MP472234BQL
CSB-EP472234BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0133 protein BCAH187_A0027 (BCAH187_A0027) CSB-YP472235BQL
CSB-EP472235BQL
CSB-BP472235BQL
CSB-MP472235BQL
CSB-EP472235BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP472236BQL
CSB-EP472236BQL
CSB-BP472236BQL
CSB-MP472236BQL
CSB-EP472236BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0297 protein BCAH187_A4520 (BCAH187_A4520) CSB-YP472237BQL
CSB-EP472237BQL
CSB-BP472237BQL
CSB-MP472237BQL
CSB-EP472237BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Quinolinate synthase A (nadA) CSB-YP472238BQL
CSB-EP472238BQL
CSB-BP472238BQL
CSB-MP472238BQL
CSB-EP472238BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable septum site-determining protein MinC (minC) CSB-YP472239BQL
CSB-EP472239BQL
CSB-BP472239BQL
CSB-MP472239BQL
CSB-EP472239BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamyl-tRNA reductase (hemA) CSB-YP472240BQL
CSB-EP472240BQL
CSB-BP472240BQL
CSB-MP472240BQL
CSB-EP472240BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative ATP:guanido phosphotransferase BCAH187_A0110 (BCAH187_A0110) CSB-YP472241BQL
CSB-EP472241BQL
CSB-BP472241BQL
CSB-MP472241BQL
CSB-EP472241BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L1 (rplA) CSB-YP472242BQL
CSB-EP472242BQL
CSB-BP472242BQL
CSB-MP472242BQL
CSB-EP472242BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S7 (rpsG) CSB-YP472243BQL
CSB-EP472243BQL
CSB-BP472243BQL
CSB-MP472243BQL
CSB-EP472243BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L4 (rplD) CSB-YP472244BQL
CSB-EP472244BQL
CSB-BP472244BQL
CSB-MP472244BQL
CSB-EP472244BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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