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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L6 (rplF) CSB-YP471263FNH
CSB-EP471263FNH
CSB-BP471263FNH
CSB-MP471263FNH
CSB-EP471263FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L36 (rpmJ) CSB-YP471264FNH
CSB-EP471264FNH
CSB-BP471264FNH
CSB-MP471264FNH
CSB-EP471264FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP471265FNH
CSB-EP471265FNH
CSB-BP471265FNH
CSB-MP471265FNH
CSB-EP471265FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L32 (rpmF) CSB-YP471266FNH
CSB-EP471266FNH
CSB-BP471266FNH
CSB-MP471266FNH
CSB-EP471266FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP471267FNH
CSB-EP471267FNH
CSB-BP471267FNH
CSB-MP471267FNH
CSB-EP471267FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 V-type ATP synthase beta chain (atpB) CSB-YP471268FNH
CSB-EP471268FNH
CSB-BP471268FNH
CSB-MP471268FNH
CSB-EP471268FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP471269FNH
CSB-EP471269FNH
CSB-BP471269FNH
CSB-MP471269FNH
CSB-EP471269FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Protease HtpX homolog (htpX), partial CSB-YP471270FNH1
CSB-EP471270FNH1
CSB-BP471270FNH1
CSB-MP471270FNH1
CSB-EP471270FNH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Tyrosine recombinase XerD-like (Spy49_0301) CSB-YP471271FNH
CSB-EP471271FNH
CSB-BP471271FNH
CSB-MP471271FNH
CSB-EP471271FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UPF0298 protein Spy49_0325 (Spy49_0325) CSB-YP471272FNH
CSB-EP471272FNH
CSB-BP471272FNH
CSB-MP471272FNH
CSB-EP471272FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP471273FNH
CSB-EP471273FNH
CSB-BP471273FNH
CSB-MP471273FNH
CSB-EP471273FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Pyrrolidone-carboxylate peptidase (pcp) CSB-YP471274FNH
CSB-EP471274FNH
CSB-BP471274FNH
CSB-MP471274FNH
CSB-EP471274FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Septation ring formation regulator EzrA (ezrA), partial CSB-YP471275FNH
CSB-EP471275FNH
CSB-BP471275FNH
CSB-MP471275FNH
CSB-EP471275FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 ATP synthase gamma chain (atpG) CSB-YP471276FNH
CSB-EP471276FNH
CSB-BP471276FNH
CSB-MP471276FNH
CSB-EP471276FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 30S ribosomal protein S21 (rpsU) CSB-YP471277FNH
CSB-EP471277FNH
CSB-BP471277FNH
CSB-MP471277FNH
CSB-EP471277FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 3-dehydroquinate dehydratase (aroD) CSB-YP471278FNH
CSB-EP471278FNH
CSB-BP471278FNH
CSB-MP471278FNH
CSB-EP471278FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP471279FNH
CSB-EP471279FNH
CSB-BP471279FNH
CSB-MP471279FNH
CSB-EP471279FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ribose-5-phosphate isomerase A (rpiA) CSB-YP471280FNH
CSB-EP471280FNH
CSB-BP471280FNH
CSB-MP471280FNH
CSB-EP471280FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP471281FNH1
CSB-EP471281FNH1
CSB-BP471281FNH1
CSB-MP471281FNH1
CSB-EP471281FNH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 GMP reductase (guaC) CSB-YP471282FNH
CSB-EP471282FNH
CSB-BP471282FNH
CSB-MP471282FNH
CSB-EP471282FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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