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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella gallinarum Chorismate synthase (aroC) CSB-YP471163SWP
CSB-EP471163SWP
CSB-BP471163SWP
CSB-MP471163SWP
CSB-EP471163SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0370 protein ypfN (ypfN), partial CSB-YP471164SWP
CSB-EP471164SWP
CSB-BP471164SWP
CSB-MP471164SWP
CSB-EP471164SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP471165SWP
CSB-EP471165SWP
CSB-BP471165SWP
CSB-MP471165SWP
CSB-EP471165SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Autonomous glycyl radical cofactor (grcA) CSB-YP471166SWP
CSB-EP471166SWP
CSB-BP471166SWP
CSB-MP471166SWP
CSB-EP471166SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein nrdI (nrdI) CSB-YP471167SWP
CSB-EP471167SWP
CSB-BP471167SWP
CSB-MP471167SWP
CSB-EP471167SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Regulatory protein recX (recX) CSB-YP471168SWP
CSB-EP471168SWP
CSB-BP471168SWP
CSB-MP471168SWP
CSB-EP471168SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Adenylyl-sulfate kinase (cysC) CSB-YP471169SWP
CSB-EP471169SWP
CSB-BP471169SWP
CSB-MP471169SWP
CSB-EP471169SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein Xni (xni) CSB-YP471170SWP
CSB-EP471170SWP
CSB-BP471170SWP
CSB-MP471170SWP
CSB-EP471170SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum DNA mismatch repair protein mutH (mutH) CSB-YP471171SWP
CSB-EP471171SWP
CSB-BP471171SWP
CSB-MP471171SWP
CSB-EP471171SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0267 protein yqfB (yqfB) CSB-YP471172SWP
CSB-EP471172SWP
CSB-BP471172SWP
CSB-MP471172SWP
CSB-EP471172SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Chromosome initiation inhibitor (iciA) CSB-YP471173SWP
CSB-EP471173SWP
CSB-BP471173SWP
CSB-MP471173SWP
CSB-EP471173SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Anti-adapter protein iraD (iraD) CSB-YP471174SWP
CSB-EP471174SWP
CSB-BP471174SWP
CSB-MP471174SWP
CSB-EP471174SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP471175SWP
CSB-EP471175SWP
CSB-BP471175SWP
CSB-MP471175SWP
CSB-EP471175SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP471176SWP
CSB-EP471176SWP
CSB-BP471176SWP
CSB-MP471176SWP
CSB-EP471176SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0306 protein yhbP (yhbP) CSB-YP471177SWP
CSB-EP471177SWP
CSB-BP471177SWP
CSB-MP471177SWP
CSB-EP471177SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP471178SWP
CSB-EP471178SWP
CSB-BP471178SWP
CSB-MP471178SWP
CSB-EP471178SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP471179SWP1
CSB-EP471179SWP1
CSB-BP471179SWP1
CSB-MP471179SWP1
CSB-EP471179SWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0412 protein yaaI (yaaI) CSB-YP471180SWP
CSB-EP471180SWP
CSB-BP471180SWP
CSB-MP471180SWP
CSB-EP471180SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Met repressor (metJ) CSB-YP471181SWP
CSB-EP471181SWP
CSB-BP471181SWP
CSB-MP471181SWP
CSB-EP471181SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Glycerol kinase (glpK), partial CSB-YP471182SWP
CSB-EP471182SWP
CSB-BP471182SWP
CSB-MP471182SWP
CSB-EP471182SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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