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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 36, mitochondrial (AIM36), partial CSB-YP471243SVH1
CSB-EP471243SVH1
CSB-BP471243SVH1
CSB-MP471243SVH1
CSB-EP471243SVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 41, mitochondrial (AIM41) CSB-YP471244SVH
CSB-EP471244SVH
CSB-BP471244SVH
CSB-MP471244SVH
CSB-EP471244SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Spindle pole component 29 (SPC29) CSB-YP471245SVH
CSB-EP471245SVH
CSB-BP471245SVH
CSB-MP471245SVH
CSB-EP471245SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Major urinary protein 17 (Mup17) CSB-YP471246MO
CSB-EP471246MO
CSB-BP471246MO
CSB-MP471246MO
CSB-EP471246MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Draxin-A CSB-YP471247SWI
CSB-EP471247SWI
CSB-BP471247SWI
CSB-MP471247SWI
CSB-EP471247SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar LYR motif-containing protein 4A (lyrm4a) CSB-YP471248SWI
CSB-EP471248SWI
CSB-BP471248SWI
CSB-MP471248SWI
CSB-EP471248SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Neuropeptide-like protein C4orf48 homolog CSB-YP471249SWI
CSB-EP471249SWI
CSB-BP471249SWI
CSB-MP471249SWI
CSB-EP471249SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Ubiquitin-like protein 4A-B (ubl4ab) CSB-YP471250SWI
CSB-EP471250SWI
CSB-BP471250SWI
CSB-MP471250SWI
CSB-EP471250SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar UPF0739 protein C1orf74 homolog CSB-YP471251SWI
CSB-EP471251SWI
CSB-BP471251SWI
CSB-MP471251SWI
CSB-EP471251SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Anamorsin-A (ciapin1-1) CSB-YP471252SWI
CSB-EP471252SWI
CSB-BP471252SWI
CSB-MP471252SWI
CSB-EP471252SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Enhancer of yellow 2 transcription factor homolog B (eny2-2) CSB-YP471253SWI
CSB-EP471253SWI
CSB-BP471253SWI
CSB-MP471253SWI
CSB-EP471253SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP471254FNH
CSB-EP471254FNH
CSB-BP471254FNH
CSB-MP471254FNH
CSB-EP471254FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Alanine racemase (alr) CSB-YP471255FNH
CSB-EP471255FNH
CSB-BP471255FNH
CSB-MP471255FNH
CSB-EP471255FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Elongation factor P (efp) CSB-YP471256FNH
CSB-EP471256FNH
CSB-BP471256FNH
CSB-MP471256FNH
CSB-EP471256FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Trigger factor (tig) CSB-YP471257FNH
CSB-EP471257FNH
CSB-BP471257FNH
CSB-MP471257FNH
CSB-EP471257FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 60 kDa chaperonin (groL), partial CSB-YP471258FNH
CSB-EP471258FNH
CSB-BP471258FNH
CSB-MP471258FNH
CSB-EP471258FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Putative competence-damage inducible protein (cinA) CSB-YP471259FNH
CSB-EP471259FNH
CSB-BP471259FNH
CSB-MP471259FNH
CSB-EP471259FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP471260FNH
CSB-EP471260FNH
CSB-BP471260FNH
CSB-MP471260FNH
CSB-EP471260FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L2 (rplB) CSB-YP471261FNH
CSB-EP471261FNH
CSB-BP471261FNH
CSB-MP471261FNH
CSB-EP471261FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 30S ribosomal protein S17 (rpsQ) CSB-YP471262FNH
CSB-EP471262FNH
CSB-BP471262FNH
CSB-MP471262FNH
CSB-EP471262FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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