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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helicobacter pylori Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP471965HUY
CSB-EP471965HUY
CSB-BP471965HUY
CSB-MP471965HUY
CSB-EP471965HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Serine--tRNA ligase (serS) CSB-YP471966HUY
CSB-EP471966HUY
CSB-BP471966HUY
CSB-MP471966HUY
CSB-EP471966HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP471967HUY
CSB-EP471967HUY
CSB-BP471967HUY
CSB-MP471967HUY
CSB-EP471967HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 10 kDa chaperonin (groS) CSB-YP471968HUY
CSB-EP471968HUY
CSB-BP471968HUY
CSB-MP471968HUY
CSB-EP471968HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Urease subunit beta (ureB), partial CSB-YP471969HUY
CSB-EP471969HUY
CSB-BP471969HUY
CSB-MP471969HUY
CSB-EP471969HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L13 (rplM) CSB-YP471970HUY
CSB-EP471970HUY
CSB-BP471970HUY
CSB-MP471970HUY
CSB-EP471970HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L20 (rplT) CSB-YP471971HUY
CSB-EP471971HUY
CSB-BP471971HUY
CSB-MP471971HUY
CSB-EP471971HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Lysine--tRNA ligase (lysS), partial CSB-YP471972HUY
CSB-EP471972HUY
CSB-BP471972HUY
CSB-MP471972HUY
CSB-EP471972HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Pentafunctional AROM polypeptide (aro1), partial CSB-YP471973SXT
CSB-EP471973SXT
CSB-BP471973SXT
CSB-MP471973SXT
CSB-EP471973SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (SJAG_01592), partial CSB-YP471974SXT
CSB-EP471974SXT
CSB-BP471974SXT
CSB-MP471974SXT
CSB-EP471974SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Methylthioribulose-1-phosphate dehydratase (mde1) CSB-YP471975SXT
CSB-EP471975SXT
CSB-BP471975SXT
CSB-MP471975SXT
CSB-EP471975SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus 40S ribosomal protein S0-C (rps0c) CSB-YP471976SXT
CSB-EP471976SXT
CSB-BP471976SXT
CSB-MP471976SXT
CSB-EP471976SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Lipoyl synthase, mitochondrial (SJAG_03553) CSB-YP471977SXT
CSB-EP471977SXT
CSB-BP471977SXT
CSB-MP471977SXT
CSB-EP471977SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus rRNA biogenesis protein rrp36 (rrp36) CSB-YP471978SXT
CSB-EP471978SXT
CSB-BP471978SXT
CSB-MP471978SXT
CSB-EP471978SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Protein get1 (get1), partial CSB-YP471979ETM1
CSB-EP471979ETM1
CSB-BP471979ETM1
CSB-MP471979ETM1
CSB-EP471979ETM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Catabolic 3-dehydroquinase (qutE) CSB-YP471980ETM
CSB-EP471980ETM
CSB-BP471980ETM
CSB-MP471980ETM
CSB-EP471980ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Probable Xaa-Pro aminopeptidase pepP (pepP) CSB-YP471981ETM
CSB-EP471981ETM
CSB-BP471981ETM
CSB-MP471981ETM
CSB-EP471981ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Adenylosuccinate synthetase (PMAA_074670) CSB-YP471982ETM
CSB-EP471982ETM
CSB-BP471982ETM
CSB-MP471982ETM
CSB-EP471982ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Required for respiratory growth protein 9, mitochondrial (rrg9) CSB-YP471983ETM
CSB-EP471983ETM
CSB-BP471983ETM
CSB-MP471983ETM
CSB-EP471983ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Altered inheritance of mitochondria protein 31, mitochondrial (aim31), partial CSB-YP471984ETM1
CSB-EP471984ETM1
CSB-BP471984ETM1
CSB-MP471984ETM1
CSB-EP471984ETM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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