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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Hut operon positive regulatory protein (hutP) CSB-YP472365BQP
CSB-EP472365BQP
CSB-BP472365BQP
CSB-MP472365BQP
CSB-EP472365BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative septation protein spoVG (spoVG) CSB-YP472366BQP
CSB-EP472366BQP
CSB-BP472366BQP
CSB-MP472366BQP
CSB-EP472366BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative ATP:guanido phosphotransferase BCG9842_B5225 (BCG9842_B5225) CSB-YP472367BQP
CSB-EP472367BQP
CSB-BP472367BQP
CSB-MP472367BQP
CSB-EP472367BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L1 (rplA) CSB-YP472368BQP
CSB-EP472368BQP
CSB-BP472368BQP
CSB-MP472368BQP
CSB-EP472368BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S12 (rpsL) CSB-YP472369BQP
CSB-EP472369BQP
CSB-BP472369BQP
CSB-MP472369BQP
CSB-EP472369BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S10 (rpsJ) CSB-YP472370BQP
CSB-EP472370BQP
CSB-BP472370BQP
CSB-MP472370BQP
CSB-EP472370BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L29 (rpmC) CSB-YP472371BQP
CSB-EP472371BQP
CSB-BP472371BQP
CSB-MP472371BQP
CSB-EP472371BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S13 (rpsM) CSB-YP472372BQP
CSB-EP472372BQP
CSB-BP472372BQP
CSB-MP472372BQP
CSB-EP472372BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Peptidase T (pepT) CSB-YP472373BQP
CSB-EP472373BQP
CSB-BP472373BQP
CSB-MP472373BQP
CSB-EP472373BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoglucosamine mutase (glmM) CSB-YP472374BQP
CSB-EP472374BQP
CSB-BP472374BQP
CSB-MP472374BQP
CSB-EP472374BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosome-binding factor A (rbfA) CSB-YP472375BQP
CSB-EP472375BQP
CSB-BP472375BQP
CSB-MP472375BQP
CSB-EP472375BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S2 (rpsB) CSB-YP472376BQP
CSB-EP472376BQP
CSB-BP472376BQP
CSB-MP472376BQP
CSB-EP472376BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease HII (rnhB) CSB-YP472377BQP
CSB-EP472377BQP
CSB-BP472377BQP
CSB-MP472377BQP
CSB-EP472377BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP472378BQP
CSB-EP472378BQP
CSB-BP472378BQP
CSB-MP472378BQP
CSB-EP472378BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP472379BQP
CSB-EP472379BQP
CSB-BP472379BQP
CSB-MP472379BQP
CSB-EP472379BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell division protein SepF (sepF) CSB-YP472380BQP
CSB-EP472380BQP
CSB-BP472380BQP
CSB-MP472380BQP
CSB-EP472380BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP472381BQP
CSB-EP472381BQP
CSB-BP472381BQP
CSB-MP472381BQP
CSB-EP472381BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Arginine deiminase (arcA) CSB-YP472382BQP
CSB-EP472382BQP
CSB-BP472382BQP
CSB-MP472382BQP
CSB-EP472382BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uncharacterized N-acetyltransferase BCG9842_B1219 (BCG9842_B1219) CSB-YP472383BQP
CSB-EP472383BQP
CSB-BP472383BQP
CSB-MP472383BQP
CSB-EP472383BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein-glutamine gamma-glutamyltransferase (tgl) CSB-YP472384BQP
CSB-EP472384BQP
CSB-BP472384BQP
CSB-MP472384BQP
CSB-EP472384BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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