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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Small, acid-soluble spore protein K (sspK) CSB-YP472165BQO
CSB-EP472165BQO
CSB-BP472165BQO
CSB-MP472165BQO
CSB-EP472165BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0295 protein BCB4264_A0544 (BCB4264_A0544), partial CSB-YP472166BQO1
CSB-EP472166BQO1
CSB-BP472166BQO1
CSB-MP472166BQO1
CSB-EP472166BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Biotin synthase (bioB) CSB-YP472167BQO
CSB-EP472167BQO
CSB-BP472167BQO
CSB-MP472167BQO
CSB-EP472167BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Acetylglutamate kinase (argB) CSB-YP472168BQO
CSB-EP472168BQO
CSB-BP472168BQO
CSB-MP472168BQO
CSB-EP472168BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP472169BQO
CSB-EP472169BQO
CSB-BP472169BQO
CSB-MP472169BQO
CSB-EP472169BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-dehydroquinate dehydratase (aroQ) CSB-YP472170BQO
CSB-EP472170BQO
CSB-BP472170BQO
CSB-MP472170BQO
CSB-EP472170BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycogen synthase (glgA) CSB-YP472171BQO
CSB-EP472171BQO
CSB-BP472171BQO
CSB-MP472171BQO
CSB-EP472171BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable cytosol aminopeptidase (pepA) CSB-YP472172BQO
CSB-EP472172BQO
CSB-BP472172BQO
CSB-MP472172BQO
CSB-EP472172BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP472173BQO
CSB-EP472173BQO
CSB-BP472173BQO
CSB-MP472173BQO
CSB-EP472173BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA mismatch repair protein mutL (mutL), partial CSB-YP472174BQO
CSB-EP472174BQO
CSB-BP472174BQO
CSB-MP472174BQO
CSB-EP472174BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP472175BQO
CSB-EP472175BQO
CSB-BP472175BQO
CSB-MP472175BQO
CSB-EP472175BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S2 (rpsB) CSB-YP472176BQO
CSB-EP472176BQO
CSB-BP472176BQO
CSB-MP472176BQO
CSB-EP472176BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L19 (rplS) CSB-YP472177BQO
CSB-EP472177BQO
CSB-BP472177BQO
CSB-MP472177BQO
CSB-EP472177BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP472178BQO
CSB-EP472178BQO
CSB-BP472178BQO
CSB-MP472178BQO
CSB-EP472178BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP472179BQO
CSB-EP472179BQO
CSB-BP472179BQO
CSB-MP472179BQO
CSB-EP472179BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Septum formation protein Maf (maf) CSB-YP472180BQO
CSB-EP472180BQO
CSB-BP472180BQO
CSB-MP472180BQO
CSB-EP472180BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Trigger factor (tig) CSB-YP472181BQO
CSB-EP472181BQO
CSB-BP472181BQO
CSB-MP472181BQO
CSB-EP472181BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoglycerate kinase (pgk) CSB-YP472182BQO
CSB-EP472182BQO
CSB-BP472182BQO
CSB-MP472182BQO
CSB-EP472182BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP472183BQO1
CSB-EP472183BQO1
CSB-BP472183BQO1
CSB-MP472183BQO1
CSB-EP472183BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0754 membrane protein BCB4264_A0915 (BCB4264_A0915), partial CSB-YP472184BQO1
CSB-EP472184BQO1
CSB-BP472184BQO1
CSB-MP472184BQO1
CSB-EP472184BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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