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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dictyoglomus thermophilum Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP471403DKI
CSB-EP471403DKI
CSB-BP471403DKI
CSB-MP471403DKI
CSB-EP471403DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum tRNA (guanine-N (1)-)-methyltransferase CSB-YP471404DKI
CSB-EP471404DKI
CSB-BP471404DKI
CSB-MP471404DKI
CSB-EP471404DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP471405TMY
CSB-EP471405TMY
CSB-BP471405TMY
CSB-MP471405TMY
CSB-EP471405TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP471406TMY
CSB-EP471406TMY
CSB-BP471406TMY
CSB-MP471406TMY
CSB-EP471406TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L6 (rplF) CSB-YP471407TMY
CSB-EP471407TMY
CSB-BP471407TMY
CSB-MP471407TMY
CSB-EP471407TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 30S ribosomal protein S17 (rpsQ) CSB-YP471408TMY
CSB-EP471408TMY
CSB-BP471408TMY
CSB-MP471408TMY
CSB-EP471408TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 30S ribosomal protein S3 (rpsC) CSB-YP471409TMY
CSB-EP471409TMY
CSB-BP471409TMY
CSB-MP471409TMY
CSB-EP471409TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L25 (rplY) CSB-YP471410TMY
CSB-EP471410TMY
CSB-BP471410TMY
CSB-MP471410TMY
CSB-EP471410TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP471411TMY
CSB-EP471411TMY
CSB-BP471411TMY
CSB-MP471411TMY
CSB-EP471411TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Heat-inducible transcription repressor HrcA (hrcA) CSB-YP471412TMY
CSB-EP471412TMY
CSB-BP471412TMY
CSB-MP471412TMY
CSB-EP471412TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP471413TMY
CSB-EP471413TMY
CSB-BP471413TMY
CSB-MP471413TMY
CSB-EP471413TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Probable transcriptional regulatory protein THEYE_A1868 (THEYE_A1868) CSB-YP471414TMY
CSB-EP471414TMY
CSB-BP471414TMY
CSB-MP471414TMY
CSB-EP471414TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Probable transaldolase (tal) CSB-YP471415TMY
CSB-EP471415TMY
CSB-BP471415TMY
CSB-MP471415TMY
CSB-EP471415TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Trigger factor (tig) CSB-YP471416TMY
CSB-EP471416TMY
CSB-BP471416TMY
CSB-MP471416TMY
CSB-EP471416TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Cysteine--tRNA ligase (cysS) CSB-YP471417TMY
CSB-EP471417TMY
CSB-BP471417TMY
CSB-MP471417TMY
CSB-EP471417TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Protein translocase subunit SecD (secD), partial CSB-YP471418TMY1
CSB-EP471418TMY1
CSB-BP471418TMY1
CSB-MP471418TMY1
CSB-EP471418TMY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L31 (rpmE) CSB-YP471419TMY
CSB-EP471419TMY
CSB-BP471419TMY
CSB-MP471419TMY
CSB-EP471419TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii DNA mismatch repair protein mutL (mutL), partial CSB-YP471420TMY
CSB-EP471420TMY
CSB-BP471420TMY
CSB-MP471420TMY
CSB-EP471420TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Elongation factor P (efp) CSB-YP471421TMY
CSB-EP471421TMY
CSB-BP471421TMY
CSB-MP471421TMY
CSB-EP471421TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L21 (rplU) CSB-YP471422TMY
CSB-EP471422TMY
CSB-BP471422TMY
CSB-MP471422TMY
CSB-EP471422TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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