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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Borrelia burgdorferi 50S ribosomal protein L9 (rplI) CSB-YP472405BUG
CSB-EP472405BUG
CSB-BP472405BUG
CSB-MP472405BUG
CSB-EP472405BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Protein RecA (recA) CSB-YP472406BUG
CSB-EP472406BUG
CSB-BP472406BUG
CSB-MP472406BUG
CSB-EP472406BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Alanine racemase (alr) CSB-YP472407BUG
CSB-EP472407BUG
CSB-BP472407BUG
CSB-MP472407BUG
CSB-EP472407BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L31 type B (rpmE2) CSB-YP472408BUG
CSB-EP472408BUG
CSB-BP472408BUG
CSB-MP472408BUG
CSB-EP472408BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi ATP-dependent protease subunit HslV (hslV) CSB-YP472409BUG
CSB-EP472409BUG
CSB-BP472409BUG
CSB-MP472409BUG
CSB-EP472409BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L13 (rplM) CSB-YP472410BUG
CSB-EP472410BUG
CSB-BP472410BUG
CSB-MP472410BUG
CSB-EP472410BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Probable M18 family aminopeptidase 1 (apeA) CSB-YP472411BUG
CSB-EP472411BUG
CSB-BP472411BUG
CSB-MP472411BUG
CSB-EP472411BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi S-ribosylhomocysteine lyase (luxS) CSB-YP472412BUG
CSB-EP472412BUG
CSB-BP472412BUG
CSB-MP472412BUG
CSB-EP472412BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L7/L12 (rplL) CSB-YP472413BUG
CSB-EP472413BUG
CSB-BP472413BUG
CSB-MP472413BUG
CSB-EP472413BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Chromosomal replication initiator protein DnaA (dnaA) CSB-YP472414BUG
CSB-EP472414BUG
CSB-BP472414BUG
CSB-MP472414BUG
CSB-EP472414BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Lipoprotein signal peptidase (lspA), partial CSB-YP472415BUG1
CSB-EP472415BUG1
CSB-BP472415BUG1
CSB-MP472415BUG1
CSB-EP472415BUG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L4 (rplD) CSB-YP472416BUG
CSB-EP472416BUG
CSB-BP472416BUG
CSB-MP472416BUG
CSB-EP472416BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 30S ribosomal protein S17 (rpsQ) CSB-YP472417BUG
CSB-EP472417BUG
CSB-BP472417BUG
CSB-MP472417BUG
CSB-EP472417BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L18 (rplR) CSB-YP472418BUG
CSB-EP472418BUG
CSB-BP472418BUG
CSB-MP472418BUG
CSB-EP472418BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 30S ribosomal protein S13 (rpsM) CSB-YP472419BUG
CSB-EP472419BUG
CSB-BP472419BUG
CSB-MP472419BUG
CSB-EP472419BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi DNA ligase (ligA), partial CSB-YP472420BUG
CSB-EP472420BUG
CSB-BP472420BUG
CSB-MP472420BUG
CSB-EP472420BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP472421BUG
CSB-EP472421BUG
CSB-BP472421BUG
CSB-MP472421BUG
CSB-EP472421BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 60 kDa chaperonin (groL), partial CSB-YP472422BUG
CSB-EP472422BUG
CSB-BP472422BUG
CSB-MP472422BUG
CSB-EP472422BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Cell division topological specificity factor (minE) CSB-YP472423AWH
CSB-EP472423AWH
CSB-BP472423AWH
CSB-MP472423AWH
CSB-EP472423AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP472424AWH
CSB-EP472424AWH
CSB-BP472424AWH
CSB-MP472424AWH
CSB-EP472424AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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