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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella agona UPF0386 protein yjhX (yjhX) CSB-YP473206SWK
CSB-EP473206SWK
CSB-BP473206SWK
CSB-MP473206SWK
CSB-EP473206SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP473207SWK
CSB-EP473207SWK
CSB-BP473207SWK
CSB-MP473207SWK
CSB-EP473207SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Tryptophan synthase beta chain (trpB) CSB-YP473208SWK
CSB-EP473208SWK
CSB-BP473208SWK
CSB-MP473208SWK
CSB-EP473208SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP473209SWK
CSB-EP473209SWK
CSB-BP473209SWK
CSB-MP473209SWK
CSB-EP473209SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP473210SWK
CSB-EP473210SWK
CSB-BP473210SWK
CSB-MP473210SWK
CSB-EP473210SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Phosphopentomutase (deoB) CSB-YP473211SWK
CSB-EP473211SWK
CSB-BP473211SWK
CSB-MP473211SWK
CSB-EP473211SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Phosphoglycerate kinase (pgk) CSB-YP473212SWK
CSB-EP473212SWK
CSB-BP473212SWK
CSB-MP473212SWK
CSB-EP473212SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Zinc transporter ZupT (zupT), partial CSB-YP473213SWK
CSB-EP473213SWK
CSB-BP473213SWK
CSB-MP473213SWK
CSB-EP473213SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP473214SWK
CSB-EP473214SWK
CSB-BP473214SWK
CSB-MP473214SWK
CSB-EP473214SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Homoserine kinase (thrB) CSB-YP473215SWK
CSB-EP473215SWK
CSB-BP473215SWK
CSB-MP473215SWK
CSB-EP473215SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Electron transport complex protein RnfC (rnfC), partial CSB-YP473216SWK
CSB-EP473216SWK
CSB-BP473216SWK
CSB-MP473216SWK
CSB-EP473216SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Multidrug resistance protein MdtK (mdtK), partial CSB-YP473217SWK
CSB-EP473217SWK
CSB-BP473217SWK
CSB-MP473217SWK
CSB-EP473217SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 5-keto-4-deoxy-D-glucarate aldolase (garL) CSB-YP473218SWK
CSB-EP473218SWK
CSB-BP473218SWK
CSB-MP473218SWK
CSB-EP473218SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribosome-binding factor A (rbfA) CSB-YP473219SWK
CSB-EP473219SWK
CSB-BP473219SWK
CSB-MP473219SWK
CSB-EP473219SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP473220SWK
CSB-EP473220SWK
CSB-BP473220SWK
CSB-MP473220SWK
CSB-EP473220SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Chaperone protein DnaJ (dnaJ) CSB-YP473221SWK
CSB-EP473221SWK
CSB-BP473221SWK
CSB-MP473221SWK
CSB-EP473221SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Crotonobetainyl-CoA dehydrogenase (caiA) CSB-YP473222SWK
CSB-EP473222SWK
CSB-BP473222SWK
CSB-MP473222SWK
CSB-EP473222SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP473223SWK
CSB-EP473223SWK
CSB-BP473223SWK
CSB-MP473223SWK
CSB-EP473223SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona L-arabinose isomerase (araA) CSB-YP473224SWK
CSB-EP473224SWK
CSB-BP473224SWK
CSB-MP473224SWK
CSB-EP473224SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Threonine--tRNA ligase (thrS) CSB-YP473225SWK
CSB-EP473225SWK
CSB-BP473225SWK
CSB-MP473225SWK
CSB-EP473225SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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