Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pelodictyon phaeoclathratiforme ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP472605ETB1
CSB-EP472605ETB1
CSB-BP472605ETB1
CSB-MP472605ETB1
CSB-EP472605ETB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Undecaprenyl-diphosphatase (uppP), partial CSB-YP472606ETB1
CSB-EP472606ETB1
CSB-BP472606ETB1
CSB-MP472606ETB1
CSB-EP472606ETB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP472607ETB
CSB-EP472607ETB
CSB-BP472607ETB
CSB-MP472607ETB
CSB-EP472607ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP472608ETB
CSB-EP472608ETB
CSB-BP472608ETB
CSB-MP472608ETB
CSB-EP472608ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Regulatory protein recX (recX) CSB-YP472609ETB
CSB-EP472609ETB
CSB-BP472609ETB
CSB-MP472609ETB
CSB-EP472609ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP472610ETB
CSB-EP472610ETB
CSB-BP472610ETB
CSB-MP472610ETB
CSB-EP472610ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP472611ETB
CSB-EP472611ETB
CSB-BP472611ETB
CSB-MP472611ETB
CSB-EP472611ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP472612ETB
CSB-EP472612ETB
CSB-BP472612ETB
CSB-MP472612ETB
CSB-EP472612ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP472613ETB
CSB-EP472613ETB
CSB-BP472613ETB
CSB-MP472613ETB
CSB-EP472613ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Enolase (eno) CSB-YP472614ETB
CSB-EP472614ETB
CSB-BP472614ETB
CSB-MP472614ETB
CSB-EP472614ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme ATP-dependent protease subunit HslV (hslV) CSB-YP472615ETB
CSB-EP472615ETB
CSB-BP472615ETB
CSB-MP472615ETB
CSB-EP472615ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Biotin synthase (bioB) CSB-YP472616ETB
CSB-EP472616ETB
CSB-BP472616ETB
CSB-MP472616ETB
CSB-EP472616ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L34 (rpmH) CSB-YP472617ETB
CSB-EP472617ETB
CSB-BP472617ETB
CSB-MP472617ETB
CSB-EP472617ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Peptide chain release factor 2 (prfB) CSB-YP472618FLY
CSB-EP472618FLY
CSB-BP472618FLY
CSB-MP472618FLY
CSB-EP472618FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia D-amino acid dehydrogenase small subunit (dadA) CSB-YP472619FLY
CSB-EP472619FLY
CSB-BP472619FLY
CSB-MP472619FLY
CSB-EP472619FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP472620FLY
CSB-EP472620FLY
CSB-BP472620FLY
CSB-MP472620FLY
CSB-EP472620FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia N utilization substance protein B homolog (nusB) CSB-YP472621FLY
CSB-EP472621FLY
CSB-BP472621FLY
CSB-MP472621FLY
CSB-EP472621FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia ATP synthase epsilon chain (atpC) CSB-YP472622FLY
CSB-EP472622FLY
CSB-BP472622FLY
CSB-MP472622FLY
CSB-EP472622FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP472623FLY1
CSB-EP472623FLY1
CSB-BP472623FLY1
CSB-MP472623FLY1
CSB-EP472623FLY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP472624FLY
CSB-EP472624FLY
CSB-BP472624FLY
CSB-MP472624FLY
CSB-EP472624FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>