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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cyanothece sp. Endonuclease V (nfi) CSB-YP472545EPW
CSB-EP472545EPW
CSB-BP472545EPW
CSB-MP472545EPW
CSB-EP472545EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. N utilization substance protein B homolog (nusB) CSB-YP472546EPW
CSB-EP472546EPW
CSB-BP472546EPW
CSB-MP472546EPW
CSB-EP472546EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Phosphomethylpyrimidine synthase (thiC) CSB-YP472547EPW
CSB-EP472547EPW
CSB-BP472547EPW
CSB-MP472547EPW
CSB-EP472547EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ribosome maturation factor RimP (rimP) CSB-YP472548EPW
CSB-EP472548EPW
CSB-BP472548EPW
CSB-MP472548EPW
CSB-EP472548EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP472549EPW
CSB-EP472549EPW
CSB-BP472549EPW
CSB-MP472549EPW
CSB-EP472549EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Cytochrome b559 subunit alpha (psbE), partial CSB-YP472550EPW1
CSB-EP472550EPW1
CSB-BP472550EPW1
CSB-MP472550EPW1
CSB-EP472550EPW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L35 (rpmI) CSB-YP472551DZZ
CSB-EP472551DZZ
CSB-BP472551DZZ
CSB-MP472551DZZ
CSB-EP472551DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP472552DZZ
CSB-EP472552DZZ
CSB-BP472552DZZ
CSB-MP472552DZZ
CSB-EP472552DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S14 (rpsN) CSB-YP472553DZZ
CSB-EP472553DZZ
CSB-BP472553DZZ
CSB-MP472553DZZ
CSB-EP472553DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L28 (rpmB) CSB-YP472554DZZ
CSB-EP472554DZZ
CSB-BP472554DZZ
CSB-MP472554DZZ
CSB-EP472554DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Photosystem I reaction center subunit VIII (psaI), partial CSB-YP472555DZZ
CSB-EP472555DZZ
CSB-BP472555DZZ
CSB-MP472555DZZ
CSB-EP472555DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP472556DZZ
CSB-EP472556DZZ
CSB-BP472556DZZ
CSB-MP472556DZZ
CSB-EP472556DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP472557DZZ
CSB-EP472557DZZ
CSB-BP472557DZZ
CSB-MP472557DZZ
CSB-EP472557DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L33 (rpmG) CSB-YP472558DZZ
CSB-EP472558DZZ
CSB-BP472558DZZ
CSB-MP472558DZZ
CSB-EP472558DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Quinolinate synthase A (nadA) CSB-YP472559DZZ
CSB-EP472559DZZ
CSB-BP472559DZZ
CSB-MP472559DZZ
CSB-EP472559DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L27 (rpmA1) CSB-YP472560AZX
CSB-EP472560AZX
CSB-BP472560AZX
CSB-MP472560AZX
CSB-EP472560AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Protein CrcB homolog (crcB), partial CSB-YP472561AZX1
CSB-EP472561AZX1
CSB-BP472561AZX1
CSB-MP472561AZX1
CSB-EP472561AZX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii 50S ribosomal protein L4 (rplD1) CSB-YP472562AZX
CSB-EP472562AZX
CSB-BP472562AZX
CSB-MP472562AZX
CSB-EP472562AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Biotin synthase (bioB) CSB-YP472563AZX
CSB-EP472563AZX
CSB-BP472563AZX
CSB-MP472563AZX
CSB-EP472563AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas macleodii Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP472564AZX
CSB-EP472564AZX
CSB-BP472564AZX
CSB-MP472564AZX
CSB-EP472564AZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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