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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Protein TusB (tusB) CSB-YP473806EOE
CSB-EP473806EOE
CSB-BP473806EOE
CSB-MP473806EOE
CSB-EP473806EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Siroheme synthase (cysG) CSB-YP473807EOE
CSB-EP473807EOE
CSB-BP473807EOE
CSB-MP473807EOE
CSB-EP473807EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 RNA 3'-terminal phosphate cyclase (rtcA) CSB-YP473808EOE
CSB-EP473808EOE
CSB-BP473808EOE
CSB-MP473808EOE
CSB-EP473808EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 FMN reductase (NADH) RutF CSB-YP473809EOE
CSB-EP473809EOE
CSB-BP473809EOE
CSB-MP473809EOE
CSB-EP473809EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP473810EOE
CSB-EP473810EOE
CSB-BP473810EOE
CSB-MP473810EOE
CSB-EP473810EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Nickel-responsive regulator (nikR) CSB-YP473811EOE
CSB-EP473811EOE
CSB-BP473811EOE
CSB-MP473811EOE
CSB-EP473811EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Urease accessory protein UreF (ureF) CSB-YP473812EOE
CSB-EP473812EOE
CSB-BP473812EOE
CSB-MP473812EOE
CSB-EP473812EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 C4-dicarboxylate transport protein (dctA), partial CSB-YP473813EOE
CSB-EP473813EOE
CSB-BP473813EOE
CSB-MP473813EOE
CSB-EP473813EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glyoxylate/hydroxypyruvate reductase A (ghrA) CSB-YP473814EOE
CSB-EP473814EOE
CSB-BP473814EOE
CSB-MP473814EOE
CSB-EP473814EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 N-methyl-L-tryptophan oxidase (solA) CSB-YP473815EOE
CSB-EP473815EOE
CSB-BP473815EOE
CSB-MP473815EOE
CSB-EP473815EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Thiamine kinase (thiK) CSB-YP473816EOE
CSB-EP473816EOE
CSB-BP473816EOE
CSB-MP473816EOE
CSB-EP473816EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP473817EOE
CSB-EP473817EOE
CSB-BP473817EOE
CSB-MP473817EOE
CSB-EP473817EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP473818EOE
CSB-EP473818EOE
CSB-BP473818EOE
CSB-MP473818EOE
CSB-EP473818EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Translocated intimin receptor Tir (tir), partial CSB-YP473819EOE
CSB-EP473819EOE
CSB-BP473819EOE
CSB-MP473819EOE
CSB-EP473819EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP473820EOE
CSB-EP473820EOE
CSB-BP473820EOE
CSB-MP473820EOE
CSB-EP473820EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP473821EOE1
CSB-EP473821EOE1
CSB-BP473821EOE1
CSB-MP473821EOE1
CSB-EP473821EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L34 (rpmH) CSB-YP473822EOE
CSB-EP473822EOE
CSB-BP473822EOE
CSB-MP473822EOE
CSB-EP473822EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 ATP synthase epsilon chain (atpC) CSB-YP473823EOE
CSB-EP473823EOE
CSB-BP473823EOE
CSB-MP473823EOE
CSB-EP473823EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Endo-type membrane-bound lytic murein transglycosylase A (emtA) CSB-YP473824EOE
CSB-EP473824EOE
CSB-BP473824EOE
CSB-MP473824EOE
CSB-EP473824EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP473825EOE
CSB-EP473825EOE
CSB-BP473825EOE
CSB-MP473825EOE
CSB-EP473825EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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