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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pyogenes serotype M49 Glutamate--tRNA ligase (gltX) CSB-YP473606FNH
CSB-EP473606FNH
CSB-BP473606FNH
CSB-MP473606FNH
CSB-EP473606FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Probable transcriptional regulatory protein Spy49_0265 (Spy49_0265) CSB-YP473607FNH
CSB-EP473607FNH
CSB-BP473607FNH
CSB-MP473607FNH
CSB-EP473607FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP473608FNH
CSB-EP473608FNH
CSB-BP473608FNH
CSB-MP473608FNH
CSB-EP473608FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Uracil phosphoribosyltransferase (upp) CSB-YP473609FNH
CSB-EP473609FNH
CSB-BP473609FNH
CSB-MP473609FNH
CSB-EP473609FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 SsrA-binding protein (smpB) CSB-YP473610FNH
CSB-EP473610FNH
CSB-BP473610FNH
CSB-MP473610FNH
CSB-EP473610FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 UPF0042 nucleotide-binding protein Spy49_0545 (Spy49_0545) CSB-YP473611FNH
CSB-EP473611FNH
CSB-BP473611FNH
CSB-MP473611FNH
CSB-EP473611FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP473612FNH
CSB-EP473612FNH
CSB-BP473612FNH
CSB-MP473612FNH
CSB-EP473612FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 50S ribosomal protein L35 (rpmI) CSB-YP473613FNH
CSB-EP473613FNH
CSB-BP473613FNH
CSB-MP473613FNH
CSB-EP473613FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Bifunctional protein pyrR (pyrR) CSB-YP473614FNH
CSB-EP473614FNH
CSB-BP473614FNH
CSB-MP473614FNH
CSB-EP473614FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ferredoxin--NADP reductase (Spy49_0668) CSB-YP473615FNH
CSB-EP473615FNH
CSB-BP473615FNH
CSB-MP473615FNH
CSB-EP473615FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Elongation factor 4 (lepA), partial CSB-YP473616FNH
CSB-EP473616FNH
CSB-BP473616FNH
CSB-MP473616FNH
CSB-EP473616FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 L-lactate dehydrogenase (ldh) CSB-YP473617FNH
CSB-EP473617FNH
CSB-BP473617FNH
CSB-MP473617FNH
CSB-EP473617FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Tyrosine recombinase XerS (xerS) CSB-YP473618FNH
CSB-EP473618FNH
CSB-BP473618FNH
CSB-MP473618FNH
CSB-EP473618FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP473619FNH
CSB-EP473619FNH
CSB-BP473619FNH
CSB-MP473619FNH
CSB-EP473619FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 D-alanine--D-alanine ligase (ddl) CSB-YP473620FNH
CSB-EP473620FNH
CSB-BP473620FNH
CSB-MP473620FNH
CSB-EP473620FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 3-dehydroquinate synthase (aroB) CSB-YP473621FNH
CSB-EP473621FNH
CSB-BP473621FNH
CSB-MP473621FNH
CSB-EP473621FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP473622FNH
CSB-EP473622FNH
CSB-BP473622FNH
CSB-MP473622FNH
CSB-EP473622FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP473623FNH
CSB-EP473623FNH
CSB-BP473623FNH
CSB-MP473623FNH
CSB-EP473623FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae L-seryl-tRNA (Sec) selenium transferase CSB-YP473624KBH
CSB-EP473624KBH
CSB-BP473624KBH
CSB-MP473624KBH
CSB-EP473624KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP473625KBH
CSB-EP473625KBH
CSB-BP473625KBH
CSB-MP473625KBH
CSB-EP473625KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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