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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Salmonella heidelberg Primosomal protein 1 (dnaT) CSB-YP472786SWQ
CSB-EP472786SWQ
CSB-BP472786SWQ
CSB-MP472786SWQ
CSB-EP472786SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg HTH-type transcriptional repressor PurR (purR) CSB-YP472787SWQ
CSB-EP472787SWQ
CSB-BP472787SWQ
CSB-MP472787SWQ
CSB-EP472787SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Electron transport complex protein RnfA (rnfA), partial CSB-YP472788SWQ1
CSB-EP472788SWQ1
CSB-BP472788SWQ1
CSB-MP472788SWQ1
CSB-EP472788SWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Probable Fe (2+)-trafficking protein CSB-YP472789SWQ
CSB-EP472789SWQ
CSB-BP472789SWQ
CSB-MP472789SWQ
CSB-EP472789SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Spermidine export protein MdtI (mdtI), partial CSB-YP472790SWQ1
CSB-EP472790SWQ1
CSB-BP472790SWQ1
CSB-MP472790SWQ1
CSB-EP472790SWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0114 protein YqhA (yqhA) CSB-YP472791SWQ
CSB-EP472791SWQ
CSB-BP472791SWQ
CSB-MP472791SWQ
CSB-EP472791SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP472792SWQ
CSB-EP472792SWQ
CSB-BP472792SWQ
CSB-MP472792SWQ
CSB-EP472792SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0246 protein yaaA (yaaA) CSB-YP472793SWQ
CSB-EP472793SWQ
CSB-BP472793SWQ
CSB-MP472793SWQ
CSB-EP472793SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Carnitine operon protein CaiE (caiE) CSB-YP472794SWQ
CSB-EP472794SWQ
CSB-BP472794SWQ
CSB-MP472794SWQ
CSB-EP472794SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP472795SWQ
CSB-EP472795SWQ
CSB-BP472795SWQ
CSB-MP472795SWQ
CSB-EP472795SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) CSB-YP472796SWQ
CSB-EP472796SWQ
CSB-BP472796SWQ
CSB-MP472796SWQ
CSB-EP472796SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 50S ribosomal protein L27 (rpmA) CSB-YP472797SWQ
CSB-EP472797SWQ
CSB-BP472797SWQ
CSB-MP472797SWQ
CSB-EP472797SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP472798SWQ
CSB-EP472798SWQ
CSB-BP472798SWQ
CSB-MP472798SWQ
CSB-EP472798SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Protein MraZ (mraZ) CSB-YP472799SWQ
CSB-EP472799SWQ
CSB-BP472799SWQ
CSB-MP472799SWQ
CSB-EP472799SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg DNA gyrase inhibitor YacG (yacG) CSB-YP472800SWQ
CSB-EP472800SWQ
CSB-BP472800SWQ
CSB-MP472800SWQ
CSB-EP472800SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP472801SWQ
CSB-EP472801SWQ
CSB-BP472801SWQ
CSB-MP472801SWQ
CSB-EP472801SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 50S ribosomal protein L17 (rplQ) CSB-YP472802SWQ
CSB-EP472802SWQ
CSB-BP472802SWQ
CSB-MP472802SWQ
CSB-EP472802SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Sugar fermentation stimulation protein A (sfsA) CSB-YP472803SWQ
CSB-EP472803SWQ
CSB-BP472803SWQ
CSB-MP472803SWQ
CSB-EP472803SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP472804SWQ
CSB-EP472804SWQ
CSB-BP472804SWQ
CSB-MP472804SWQ
CSB-EP472804SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0253 protein yaeP (yaeP) CSB-YP472805SWQ
CSB-EP472805SWQ
CSB-BP472805SWQ
CSB-MP472805SWQ
CSB-EP472805SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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