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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Salmonella schwarzengrund Adenylosuccinate synthetase (purA) CSB-YP472846SWV
CSB-EP472846SWV
CSB-BP472846SWV
CSB-MP472846SWV
CSB-EP472846SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Cell division inhibitor SulA (sulA) CSB-YP472847SWV
CSB-EP472847SWV
CSB-BP472847SWV
CSB-MP472847SWV
CSB-EP472847SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Chaperone modulatory protein CbpM (cbpM) CSB-YP472848SWV
CSB-EP472848SWV
CSB-BP472848SWV
CSB-MP472848SWV
CSB-EP472848SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glucans biosynthesis protein C (mdoC), partial CSB-YP472849SWV
CSB-EP472849SWV
CSB-BP472849SWV
CSB-MP472849SWV
CSB-EP472849SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glutamate--cysteine ligase (gshA), partial CSB-YP472850SWV
CSB-EP472850SWV
CSB-BP472850SWV
CSB-MP472850SWV
CSB-EP472850SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund L-ribulose-5-phosphate 3-epimerase UlaE (ulaE) CSB-YP472851SWV
CSB-EP472851SWV
CSB-BP472851SWV
CSB-MP472851SWV
CSB-EP472851SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Multidrug resistance protein MdtH (mdtH), partial CSB-YP472852SWV
CSB-EP472852SWV
CSB-BP472852SWV
CSB-MP472852SWV
CSB-EP472852SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Beta-hexosaminidase (nagZ) CSB-YP472853SWV
CSB-EP472853SWV
CSB-BP472853SWV
CSB-MP472853SWV
CSB-EP472853SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP472854SWV
CSB-EP472854SWV
CSB-BP472854SWV
CSB-MP472854SWV
CSB-EP472854SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP472855SWV
CSB-EP472855SWV
CSB-BP472855SWV
CSB-MP472855SWV
CSB-EP472855SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Primosomal protein 1 (dnaT) CSB-YP472856SWV
CSB-EP472856SWV
CSB-BP472856SWV
CSB-MP472856SWV
CSB-EP472856SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Phosphopentomutase (deoB) CSB-YP472857SWV
CSB-EP472857SWV
CSB-BP472857SWV
CSB-MP472857SWV
CSB-EP472857SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0229 protein yeaH (yeaH) CSB-YP472858SWV
CSB-EP472858SWV
CSB-BP472858SWV
CSB-MP472858SWV
CSB-EP472858SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Succinylglutamate desuccinylase (astE) CSB-YP472859SWV
CSB-EP472859SWV
CSB-BP472859SWV
CSB-MP472859SWV
CSB-EP472859SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L35 (rpmI) CSB-YP472860SWV
CSB-EP472860SWV
CSB-BP472860SWV
CSB-MP472860SWV
CSB-EP472860SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0061 protein ydiU (ydiU) CSB-YP472861SWV
CSB-EP472861SWV
CSB-BP472861SWV
CSB-MP472861SWV
CSB-EP472861SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP472862SWV1
CSB-EP472862SWV1
CSB-BP472862SWV1
CSB-MP472862SWV1
CSB-EP472862SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Multidrug resistance protein MdtK (mdtK), partial CSB-YP472863SWV
CSB-EP472863SWV
CSB-BP472863SWV
CSB-MP472863SWV
CSB-EP472863SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Electron transport complex protein RnfC (rnfC), partial CSB-YP472864SWV
CSB-EP472864SWV
CSB-BP472864SWV
CSB-MP472864SWV
CSB-EP472864SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Arginine exporter protein ArgO (argO), partial CSB-YP472865SWV1
CSB-EP472865SWV1
CSB-BP472865SWV1
CSB-MP472865SWV1
CSB-EP472865SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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