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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella agona Pantothenate kinase (coaA) CSB-YP473186SWK
CSB-EP473186SWK
CSB-BP473186SWK
CSB-MP473186SWK
CSB-EP473186SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP473187SWK
CSB-EP473187SWK
CSB-BP473187SWK
CSB-MP473187SWK
CSB-EP473187SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Uncharacterized MFS-type transporter ycaD (ycaD), partial CSB-YP473188SWK
CSB-EP473188SWK
CSB-BP473188SWK
CSB-MP473188SWK
CSB-EP473188SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP473189SWK
CSB-EP473189SWK
CSB-BP473189SWK
CSB-MP473189SWK
CSB-EP473189SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Chaperone protein hscA (hscA), partial CSB-YP473190SWK
CSB-EP473190SWK
CSB-BP473190SWK
CSB-MP473190SWK
CSB-EP473190SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP473191SWK
CSB-EP473191SWK
CSB-BP473191SWK
CSB-MP473191SWK
CSB-EP473191SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP473192SWK1
CSB-EP473192SWK1
CSB-BP473192SWK1
CSB-MP473192SWK1
CSB-EP473192SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP473193SWK
CSB-EP473193SWK
CSB-BP473193SWK
CSB-MP473193SWK
CSB-EP473193SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP473194SWK
CSB-EP473194SWK
CSB-BP473194SWK
CSB-MP473194SWK
CSB-EP473194SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 30S ribosomal protein S16 (rpsP) CSB-YP473195SWK
CSB-EP473195SWK
CSB-BP473195SWK
CSB-MP473195SWK
CSB-EP473195SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Cytochrome c-552 (nrfA) CSB-YP473196SWK
CSB-EP473196SWK
CSB-BP473196SWK
CSB-MP473196SWK
CSB-EP473196SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Uncharacterized protein YjeA (yjeA) CSB-YP473197SWK
CSB-EP473197SWK
CSB-BP473197SWK
CSB-MP473197SWK
CSB-EP473197SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Glutamate--cysteine ligase (gshA), partial CSB-YP473198SWK
CSB-EP473198SWK
CSB-BP473198SWK
CSB-MP473198SWK
CSB-EP473198SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP473199SWK
CSB-EP473199SWK
CSB-BP473199SWK
CSB-MP473199SWK
CSB-EP473199SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona HTH-type transcriptional regulator UlaR (ulaR) CSB-YP473200SWK
CSB-EP473200SWK
CSB-BP473200SWK
CSB-MP473200SWK
CSB-EP473200SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP473201SWK
CSB-EP473201SWK
CSB-BP473201SWK
CSB-MP473201SWK
CSB-EP473201SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP473202SWK
CSB-EP473202SWK
CSB-BP473202SWK
CSB-MP473202SWK
CSB-EP473202SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0059 membrane protein yebN (yebN), partial CSB-YP473203SWK1
CSB-EP473203SWK1
CSB-BP473203SWK1
CSB-MP473203SWK1
CSB-EP473203SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0745 protein YcgL (ycgL) CSB-YP473204SWK
CSB-EP473204SWK
CSB-BP473204SWK
CSB-MP473204SWK
CSB-EP473204SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Cell division protein FtsB (ftsB), partial CSB-YP473205SWK
CSB-EP473205SWK
CSB-BP473205SWK
CSB-MP473205SWK
CSB-EP473205SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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