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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Homoserine O-succinyltransferase (metA) CSB-YP473846EOE
CSB-EP473846EOE
CSB-BP473846EOE
CSB-MP473846EOE
CSB-EP473846EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP473847EOE
CSB-EP473847EOE
CSB-BP473847EOE
CSB-MP473847EOE
CSB-EP473847EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S2 (rpsB) CSB-YP473848EOE
CSB-EP473848EOE
CSB-BP473848EOE
CSB-MP473848EOE
CSB-EP473848EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0294 protein yafD (yafD) CSB-YP473849EOE
CSB-EP473849EOE
CSB-BP473849EOE
CSB-MP473849EOE
CSB-EP473849EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Gamma-aminobutyraldehyde dehydrogenase (prr) CSB-YP473850EOE
CSB-EP473850EOE
CSB-BP473850EOE
CSB-MP473850EOE
CSB-EP473850EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP473851EOE
CSB-EP473851EOE
CSB-BP473851EOE
CSB-MP473851EOE
CSB-EP473851EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Co-chaperone protein hscB (hscB) CSB-YP473852EOE
CSB-EP473852EOE
CSB-BP473852EOE
CSB-MP473852EOE
CSB-EP473852EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 DNA repair protein recO (recO) CSB-YP473853EOE
CSB-EP473853EOE
CSB-BP473853EOE
CSB-MP473853EOE
CSB-EP473853EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Autonomous glycyl radical cofactor (grcA) CSB-YP473854EOE
CSB-EP473854EOE
CSB-BP473854EOE
CSB-MP473854EOE
CSB-EP473854EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cation/acetate symporter ActP (actP), partial CSB-YP473855EOE
CSB-EP473855EOE
CSB-BP473855EOE
CSB-MP473855EOE
CSB-EP473855EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 HTH-type transcriptional regulator BetI (betI) CSB-YP473856EOE
CSB-EP473856EOE
CSB-BP473856EOE
CSB-MP473856EOE
CSB-EP473856EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S16 (rpsP) CSB-YP473857EOE
CSB-EP473857EOE
CSB-BP473857EOE
CSB-MP473857EOE
CSB-EP473857EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein CsiD (csiD) CSB-YP473858EOE
CSB-EP473858EOE
CSB-BP473858EOE
CSB-MP473858EOE
CSB-EP473858EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Uncharacterized protein YjeA (yjeA) CSB-YP473859EOE
CSB-EP473859EOE
CSB-BP473859EOE
CSB-MP473859EOE
CSB-EP473859EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 HTH-type transcriptional regulator UlaR (ulaR) CSB-YP473860EOE
CSB-EP473860EOE
CSB-BP473860EOE
CSB-MP473860EOE
CSB-EP473860EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase CSB-YP473861EOE
CSB-EP473861EOE
CSB-BP473861EOE
CSB-MP473861EOE
CSB-EP473861EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0178 protein yaiI (yaiI) CSB-YP473862EOE
CSB-EP473862EOE
CSB-BP473862EOE
CSB-MP473862EOE
CSB-EP473862EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein syd (syd) CSB-YP473863EOE
CSB-EP473863EOE
CSB-BP473863EOE
CSB-MP473863EOE
CSB-EP473863EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0307 protein YjgA (yjgA) CSB-YP473864EOE
CSB-EP473864EOE
CSB-BP473864EOE
CSB-MP473864EOE
CSB-EP473864EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Anti-adapter protein iraD (iraD) CSB-YP473865EOE
CSB-EP473865EOE
CSB-BP473865EOE
CSB-MP473865EOE
CSB-EP473865EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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