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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae serotype 19F Indole-3-glycerol phosphate synthase (trpC) CSB-YP474846FNE
CSB-EP474846FNE
CSB-BP474846FNE
CSB-MP474846FNE
CSB-EP474846FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP474847GFN
CSB-EP474847GFN
CSB-BP474847GFN
CSB-MP474847GFN
CSB-EP474847GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis UPF0502 protein Gbem_0102 (Gbem_0102) CSB-YP474848GFN
CSB-EP474848GFN
CSB-BP474848GFN
CSB-MP474848GFN
CSB-EP474848GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis UPF0391 membrane protein Gbem_0127 (Gbem_0127), partial CSB-YP474849GFN
CSB-EP474849GFN
CSB-BP474849GFN
CSB-MP474849GFN
CSB-EP474849GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Recombination protein RecR (recR) CSB-YP474850GFN
CSB-EP474850GFN
CSB-BP474850GFN
CSB-MP474850GFN
CSB-EP474850GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP474851GFN
CSB-EP474851GFN
CSB-BP474851GFN
CSB-MP474851GFN
CSB-EP474851GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 10 kDa chaperonin (groS) CSB-YP474852GFN
CSB-EP474852GFN
CSB-BP474852GFN
CSB-MP474852GFN
CSB-EP474852GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Tryptophan synthase alpha chain (trpA) CSB-YP474853GFN
CSB-EP474853GFN
CSB-BP474853GFN
CSB-MP474853GFN
CSB-EP474853GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Elongation factor 4 (lepA), partial CSB-YP474854GFN
CSB-EP474854GFN
CSB-BP474854GFN
CSB-MP474854GFN
CSB-EP474854GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP474855GFN
CSB-EP474855GFN
CSB-BP474855GFN
CSB-MP474855GFN
CSB-EP474855GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP474856GFN
CSB-EP474856GFN
CSB-BP474856GFN
CSB-MP474856GFN
CSB-EP474856GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Protein grpE (grpE) CSB-YP474857GFN
CSB-EP474857GFN
CSB-BP474857GFN
CSB-MP474857GFN
CSB-EP474857GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Biotin synthase (bioB) CSB-YP474858GFN
CSB-EP474858GFN
CSB-BP474858GFN
CSB-MP474858GFN
CSB-EP474858GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP474859GFN
CSB-EP474859GFN
CSB-BP474859GFN
CSB-MP474859GFN
CSB-EP474859GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis UPF0234 protein Gbem_0619 (Gbem_0619) CSB-YP474860GFN
CSB-EP474860GFN
CSB-BP474860GFN
CSB-MP474860GFN
CSB-EP474860GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 7-cyano-7-deazaguanine synthase (queC) CSB-YP474861GFN
CSB-EP474861GFN
CSB-BP474861GFN
CSB-MP474861GFN
CSB-EP474861GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP474862GFN
CSB-EP474862GFN
CSB-BP474862GFN
CSB-MP474862GFN
CSB-EP474862GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis DNA gyrase inhibitor YacG (yacG) CSB-YP474863GFN
CSB-EP474863GFN
CSB-BP474863GFN
CSB-MP474863GFN
CSB-EP474863GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Gamma-glutamyl phosphate reductase (proA) CSB-YP474864GFN
CSB-EP474864GFN
CSB-BP474864GFN
CSB-MP474864GFN
CSB-EP474864GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L33 (rpmG) CSB-YP474865GFN
CSB-EP474865GFN
CSB-BP474865GFN
CSB-MP474865GFN
CSB-EP474865GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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