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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella gallinarum HTH-type transcriptional regulator iscR (iscR) CSB-YP475246SWP
CSB-EP475246SWP
CSB-BP475246SWP
CSB-MP475246SWP
CSB-EP475246SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 30S ribosomal protein S16 (rpsP) CSB-YP475247SWP
CSB-EP475247SWP
CSB-BP475247SWP
CSB-MP475247SWP
CSB-EP475247SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum S-ribosylhomocysteine lyase (luxS) CSB-YP475248SWP
CSB-EP475248SWP
CSB-BP475248SWP
CSB-MP475248SWP
CSB-EP475248SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Anaerobic nitric oxide reductase transcription regulator norR (norR), partial CSB-YP475249SWP
CSB-EP475249SWP
CSB-BP475249SWP
CSB-MP475249SWP
CSB-EP475249SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP475250SWP
CSB-EP475250SWP
CSB-BP475250SWP
CSB-MP475250SWP
CSB-EP475250SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein syd (syd) CSB-YP475251SWP
CSB-EP475251SWP
CSB-BP475251SWP
CSB-MP475251SWP
CSB-EP475251SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Lysophospholipid transporter lplT (lplT), partial CSB-YP475252SWP
CSB-EP475252SWP
CSB-BP475252SWP
CSB-MP475252SWP
CSB-EP475252SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Cell division protein ZapA (zapA) CSB-YP475253SWP
CSB-EP475253SWP
CSB-BP475253SWP
CSB-MP475253SWP
CSB-EP475253SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein sprT (sprT) CSB-YP475254SWP
CSB-EP475254SWP
CSB-BP475254SWP
CSB-MP475254SWP
CSB-EP475254SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Uronate isomerase (uxaC) CSB-YP475255SWP
CSB-EP475255SWP
CSB-BP475255SWP
CSB-MP475255SWP
CSB-EP475255SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 30S ribosomal protein S21 (rpsU) CSB-YP475256SWP
CSB-EP475256SWP
CSB-BP475256SWP
CSB-MP475256SWP
CSB-EP475256SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 5-keto-4-deoxy-D-glucarate aldolase (garL) CSB-YP475257SWP
CSB-EP475257SWP
CSB-BP475257SWP
CSB-MP475257SWP
CSB-EP475257SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 30S ribosomal protein S15 (rpsO) CSB-YP475258SWP
CSB-EP475258SWP
CSB-BP475258SWP
CSB-MP475258SWP
CSB-EP475258SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum GTPase obg (obg) CSB-YP475259SWP
CSB-EP475259SWP
CSB-BP475259SWP
CSB-MP475259SWP
CSB-EP475259SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP475260SWP
CSB-EP475260SWP
CSB-BP475260SWP
CSB-MP475260SWP
CSB-EP475260SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein AaeX (aaeX), partial CSB-YP475261SWP1
CSB-EP475261SWP1
CSB-BP475261SWP1
CSB-MP475261SWP1
CSB-EP475261SWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP475262SWP
CSB-EP475262SWP
CSB-BP475262SWP
CSB-MP475262SWP
CSB-EP475262SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 30S ribosomal protein S20 (rpsT) CSB-YP475263SWP
CSB-EP475263SWP
CSB-BP475263SWP
CSB-MP475263SWP
CSB-EP475263SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Probable fructose-6-phosphate aldolase (fsa) CSB-YP475264SWP
CSB-EP475264SWP
CSB-BP475264SWP
CSB-MP475264SWP
CSB-EP475264SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Cell division protein ZapB (zapB) CSB-YP475265SWP
CSB-EP475265SWP
CSB-BP475265SWP
CSB-MP475265SWP
CSB-EP475265SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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