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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella gallinarum Protein translocase subunit SecA (secA), partial CSB-YP473546SWP
CSB-EP473546SWP
CSB-BP473546SWP
CSB-MP473546SWP
CSB-EP473546SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Aspartate 1-decarboxylase (panD) CSB-YP473547SWP
CSB-EP473547SWP
CSB-BP473547SWP
CSB-MP473547SWP
CSB-EP473547SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP473548SWP
CSB-EP473548SWP
CSB-BP473548SWP
CSB-MP473548SWP
CSB-EP473548SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP473549SWP
CSB-EP473549SWP
CSB-BP473549SWP
CSB-MP473549SWP
CSB-EP473549SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Phosphoglycerate kinase (pgk) CSB-YP473550BUH
CSB-EP473550BUH
CSB-BP473550BUH
CSB-MP473550BUH
CSB-EP473550BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Glycerol kinase (glpK) CSB-YP473551BUH
CSB-EP473551BUH
CSB-BP473551BUH
CSB-MP473551BUH
CSB-EP473551BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii V-type proton ATPase subunit E (atpE) CSB-YP473552BUH
CSB-EP473552BUH
CSB-BP473552BUH
CSB-MP473552BUH
CSB-EP473552BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 30S ribosomal protein S18 (rpsR) CSB-YP473553BUH
CSB-EP473553BUH
CSB-BP473553BUH
CSB-MP473553BUH
CSB-EP473553BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Enolase (eno) CSB-YP473554BUH
CSB-EP473554BUH
CSB-BP473554BUH
CSB-MP473554BUH
CSB-EP473554BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L10 (rplJ) CSB-YP473555BUH
CSB-EP473555BUH
CSB-BP473555BUH
CSB-MP473555BUH
CSB-EP473555BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L34 (rpmH) CSB-YP473556BUH
CSB-EP473556BUH
CSB-BP473556BUH
CSB-MP473556BUH
CSB-EP473556BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii UPF0133 protein BDU_464 (BDU_464) CSB-YP473557BUH
CSB-EP473557BUH
CSB-BP473557BUH
CSB-MP473557BUH
CSB-EP473557BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L14 (rplN) CSB-YP473558BUH
CSB-EP473558BUH
CSB-BP473558BUH
CSB-MP473558BUH
CSB-EP473558BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 30S ribosomal protein S13 (rpsM) CSB-YP473559BUH
CSB-EP473559BUH
CSB-BP473559BUH
CSB-MP473559BUH
CSB-EP473559BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Arginine--tRNA ligase (argS), partial CSB-YP473560BUH
CSB-EP473560BUH
CSB-BP473560BUH
CSB-MP473560BUH
CSB-EP473560BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Acetate kinase (ackA) CSB-YP473561BUH
CSB-EP473561BUH
CSB-BP473561BUH
CSB-MP473561BUH
CSB-EP473561BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Ribonuclease Z (rnz) CSB-YP473562BUH
CSB-EP473562BUH
CSB-BP473562BUH
CSB-MP473562BUH
CSB-EP473562BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L3 (rplC) CSB-YP473563BUK
CSB-EP473563BUK
CSB-BP473563BUK
CSB-MP473563BUK
CSB-EP473563BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 30S ribosomal protein S17 (rpsQ) CSB-YP473564BUK
CSB-EP473564BUK
CSB-BP473564BUK
CSB-MP473564BUK
CSB-EP473564BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L36 (rpmJ) CSB-YP473565BUK
CSB-EP473565BUK
CSB-BP473565BUK
CSB-MP473565BUK
CSB-EP473565BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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