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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Borrelia recurrentis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP473566BUK
CSB-EP473566BUK
CSB-BP473566BUK
CSB-MP473566BUK
CSB-EP473566BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Phosphopantetheine adenylyltransferase (coaD) CSB-YP473567BUK
CSB-EP473567BUK
CSB-BP473567BUK
CSB-MP473567BUK
CSB-EP473567BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L25 (rplY) CSB-YP473568BUK
CSB-EP473568BUK
CSB-BP473568BUK
CSB-MP473568BUK
CSB-EP473568BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Uridine kinase (udk) CSB-YP473569BUK
CSB-EP473569BUK
CSB-BP473569BUK
CSB-MP473569BUK
CSB-EP473569BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP473570BUK
CSB-EP473570BUK
CSB-BP473570BUK
CSB-MP473570BUK
CSB-EP473570BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Carbon storage regulator homolog (csrA) CSB-YP473571BUK
CSB-EP473571BUK
CSB-BP473571BUK
CSB-MP473571BUK
CSB-EP473571BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Serine--tRNA ligase (serS) CSB-YP473572BUK
CSB-EP473572BUK
CSB-BP473572BUK
CSB-MP473572BUK
CSB-EP473572BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L13 (rplM) CSB-YP473573BUK
CSB-EP473573BUK
CSB-BP473573BUK
CSB-MP473573BUK
CSB-EP473573BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis 50S ribosomal protein L33 (rpmG) CSB-YP473574BUK
CSB-EP473574BUK
CSB-BP473574BUK
CSB-MP473574BUK
CSB-EP473574BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia recurrentis Membrane protein insertase YidC (yidC) CSB-YP473575BUK
CSB-EP473575BUK
CSB-BP473575BUK
CSB-MP473575BUK
CSB-EP473575BUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Enhancer of translation termination 1 (ETT1) CSB-YP473576DIS
CSB-EP473576DIS
CSB-BP473576DIS
CSB-MP473576DIS
CSB-EP473576DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Glutamyl-tRNA (Gln) amidotransferase subunit B, mitochondrial, partial CSB-YP473577SVH
CSB-EP473577SVH
CSB-BP473577SVH
CSB-MP473577SVH
CSB-EP473577SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Spindle pole body component KRE28 (KRE28) CSB-YP473578SVH
CSB-EP473578SVH
CSB-BP473578SVH
CSB-MP473578SVH
CSB-EP473578SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcriptional activator FLO8 (FLO8), partial CSB-YP473579SVH
CSB-EP473579SVH
CSB-BP473579SVH
CSB-MP473579SVH
CSB-EP473579SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Probable endonuclease LCL3 (LCL3), partial CSB-YP473580SVH1
CSB-EP473580SVH1
CSB-BP473580SVH1
CSB-MP473580SVH1
CSB-EP473580SVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Topoisomerase I damage affected protein 11 (TDA11) CSB-YP473581SVH
CSB-EP473581SVH
CSB-BP473581SVH
CSB-MP473581SVH
CSB-EP473581SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Autophagy-related protein 32 (ATG32), partial CSB-YP473582SVH1
CSB-EP473582SVH1
CSB-BP473582SVH1
CSB-MP473582SVH1
CSB-EP473582SVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 23, mitochondrial (AIM23) CSB-YP473583SVH
CSB-EP473583SVH
CSB-BP473583SVH
CSB-MP473583SVH
CSB-EP473583SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Maintenance of telomere capping protein 2 (MTC2) CSB-YP473584SVH
CSB-EP473584SVH
CSB-BP473584SVH
CSB-MP473584SVH
CSB-EP473584SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein UTH1 (UTH1) CSB-YP473585SVH
CSB-EP473585SVH
CSB-BP473585SVH
CSB-MP473585SVH
CSB-EP473585SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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