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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L33 (rpmG) CSB-YP474346ETB
CSB-EP474346ETB
CSB-BP474346ETB
CSB-MP474346ETB
CSB-EP474346ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Putative pterin-4-alpha-carbinolamine dehydratase (Ppha_0103) CSB-YP474347ETB
CSB-EP474347ETB
CSB-BP474347ETB
CSB-MP474347ETB
CSB-EP474347ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Phosphomethylpyrimidine synthase (thiC), partial CSB-YP474348ETB
CSB-EP474348ETB
CSB-BP474348ETB
CSB-MP474348ETB
CSB-EP474348ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP474349ETB
CSB-EP474349ETB
CSB-BP474349ETB
CSB-MP474349ETB
CSB-EP474349ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L3 (rplC) CSB-YP474350ETB
CSB-EP474350ETB
CSB-BP474350ETB
CSB-MP474350ETB
CSB-EP474350ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L24 (rplX) CSB-YP474351ETB
CSB-EP474351ETB
CSB-BP474351ETB
CSB-MP474351ETB
CSB-EP474351ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 30S ribosomal protein S5 (rpsE) CSB-YP474352ETB
CSB-EP474352ETB
CSB-BP474352ETB
CSB-MP474352ETB
CSB-EP474352ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP474353ETB
CSB-EP474353ETB
CSB-BP474353ETB
CSB-MP474353ETB
CSB-EP474353ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Light-independent protochlorophyllide reductase subunit B (bchB), partial CSB-YP474354ETB
CSB-EP474354ETB
CSB-BP474354ETB
CSB-MP474354ETB
CSB-EP474354ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Histidine--tRNA ligase (hisS) CSB-YP474355ETB
CSB-EP474355ETB
CSB-BP474355ETB
CSB-MP474355ETB
CSB-EP474355ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP474356ETB
CSB-EP474356ETB
CSB-BP474356ETB
CSB-MP474356ETB
CSB-EP474356ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Glutamate--tRNA ligase (gltX), partial CSB-YP474357ETB
CSB-EP474357ETB
CSB-BP474357ETB
CSB-MP474357ETB
CSB-EP474357ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP474358ETB
CSB-EP474358ETB
CSB-BP474358ETB
CSB-MP474358ETB
CSB-EP474358ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Ketol-acid reductoisomerase (ilvC) CSB-YP474359ETB
CSB-EP474359ETB
CSB-BP474359ETB
CSB-MP474359ETB
CSB-EP474359ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP474360ETB
CSB-EP474360ETB
CSB-BP474360ETB
CSB-MP474360ETB
CSB-EP474360ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Pantothenate synthetase (panC) CSB-YP474361ETB
CSB-EP474361ETB
CSB-BP474361ETB
CSB-MP474361ETB
CSB-EP474361ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Sec-independent protein translocase protein TatA (tatA), partial CSB-YP474362ETB
CSB-EP474362ETB
CSB-BP474362ETB
CSB-MP474362ETB
CSB-EP474362ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Tryptophan synthase alpha chain (trpA) CSB-YP474363ETB
CSB-EP474363ETB
CSB-BP474363ETB
CSB-MP474363ETB
CSB-EP474363ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP474364ETB
CSB-EP474364ETB
CSB-BP474364ETB
CSB-MP474364ETB
CSB-EP474364ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme 50S ribosomal protein L27 (rpmA) CSB-YP474365ETB
CSB-EP474365ETB
CSB-BP474365ETB
CSB-MP474365ETB
CSB-EP474365ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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